中药方剂益肾汤对IgA肾病模型小鼠肾组织表达MMP-9、TIMP-1的影响

目的：探讨中药“益肾汤”治疗IgA肾病（IgAN）的机制。方法：用“口服牛血清白蛋白联合尾静脉注射葡萄球菌肠毒素B”法复制小鼠IgAN模型。设正常照、模型照、益肾汤低浓度和高浓度组共4组。FQ-RT-PCR法检测小鼠肾组织MMP-9、TIMP-1mRNA表达、免疫组化SABC法检测小鼠肾组织MMP-9、TIMP-1的含量。结果：模型组小鼠肾间质MMP-9表达与正常组无统计学意义,而模型组TIMP-1表达则明显上调,有统计学意义（P〈0.05）。益肾汤低浓度与高浓度组之间肾小管间质MMP-9、TIMP-1表达无统计学意义,但两组MMP-9表达均强于模型组（P〈0.05）,而TIMP-1的表达均弱于模型组（P〈0.05）。结论：益肾汤可促进小鼠肾小管间质MMP-9的表达、抑制TIMP-1的表达、促进ECM的降解,进而延缓IgAN肾小球硬化的进展。

The effects of TCM Yishen Decoction on the expression of MMP-9 and TIMP-1 in kidney of mouse with IgA nephropathy

AIM:To explore effects of Yishen Decoction on the expression of MMP-9 and TIMP-1 in kidney of mice with IgA nephropathy(IgAN).METHODS:The IgAN model was established by oral administration of bovine serum albumin(BSA) together with the injection of Staphylococcus enterotoxin B(SEB) through caudal vein.The mRNA and protein expression of MMP-9 and TIMP-1 were measured by fluorescent quantitative RT-PCR and immunohistochemistry respectively.RESULTS:No significant differences of mRNA and protein expression of MMP-9 and TIMP-1 were found between the low and high concentration Yishen Decoction treated group.But the expression of TIMP-1 in the Yishen Decoction treated group was lower than that in the IgAN group,while that of MMP-9 was higher than that in the control group.CONCLUSION:The TCM Yishen Decoction can inhibit the abnormal expression of TIMP-1,and strengthen the expression of MMP-9 in kidney of mouse with IgA nephropathy.