| 肿瘤-睾丸抗原MAGE-1和NY-ESO-1 mRNA在胃肠间质瘤中的表达 |
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| 引用本文： | 赖斌,朱培谦,朱胜昌,罗洪亮. 肿瘤-睾丸抗原MAGE-1和NY-ESO-1 mRNA在胃肠间质瘤中的表达[J]. 世界华人消化杂志, 2010, 18(4) |
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| 作者姓名： | 赖斌 朱培谦 朱胜昌 罗洪亮 |
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| 作者单位： | 南昌大学第二附属医院胃肠外科,江西省南昌市,330006;宜春市人民医院普外科,江西省宜春市,336000 |
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| 摘 要： | 目的:探讨肿瘤-睾丸抗原(CTA)MAGE-1和NY-ESO-1作为胃肠间质瘤(GISTs)免疫治疗特异性靶点及MAGE-1和NY-ESO-1 mRNA作为辅助GISTs危险度分级指标的可能性.及其与GISTs生物学行为的关系.方法:采用逆转录-聚合酶链反应(RT-PCR)技术,检测30例GISTs中MAGE-1和NY-ESO-1mRNA的表达,并取正常胃肠道组织作为阴性对照组,同时分析MAGE-1和NY-ESO-1mRNA表达与病理特征的关系.结果:正常对照组中无阳性表达,30例GISTs中,18例至少表达一种CTA,MAGE-1和NY-ESO-1 mRNA在GISTs中的表达率分别为30%和47%.MAGE-1和NY-ESO-1 mRNA表达与患者年龄、性别和病理类型无关,而与肿瘤生长部位、肿瘤大小及危险度分级有关(P<0.05).MAGE-1和NY-ESO-1 mRNA在低危、中危、高危三个组中表达量随着危险度分级的升高而增高,三组间差异有统计学意义(P<0.05).MAGE-1和NY-ESO-1 mRNA在GISTs中的表达不存在相关性(r=0.018,P>0.05).结论:MAGE-1和NY-ESO-1 mRNA在GISTs中的高特异性表达,其抗原有望成为GISTs免疫治疗特异性的靶点:MAGE-1和NY-ESO-1 mRNA表达与GISTs危险度分级有关,MAGE-1和NY-ESO-1 mRNA有望成为辅助GISTs危险度分级的诊断指标.
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| 关 键 词： | 胃肠道间质瘤 肿瘤-睾丸抗原 逆转录-聚合酶链反应 MAGE-1 NY-ESO-1 |
Clinical implications of expression of MAGE-1 and NY-ESO-1 mRNAs in gastrointestinal stromal tumors |
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| Bin Lai,Pei-Qian Zhu,Sheng-Chang Zhu,Hong-Liang Luo. Clinical implications of expression of MAGE-1 and NY-ESO-1 mRNAs in gastrointestinal stromal tumors[J]. World Chinese Journal of Digestology, 2010, 18(4) |
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| Authors: | Bin Lai Pei-Qian Zhu Sheng-Chang Zhu Hong-Liang Luo |
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| Affiliation: | Bin Lai,Pei-Qian Zhu,Hong-Liang Luo,Department of Gastrointestinal Surgery,the Second Affiliated Hospital to Nanchang University,Nanchang 330006,Jiangxi Province,China Sheng-Chang Zhu,Department of General Surgery,the People's Hospital of Yichun City,Yichun 336000,China |
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| Abstract: | AIM:To evaluate the possibility of using melanoma antigen-1(MAGE-1)and New Yorkesophageal-1(NY-ESO-1)antigens as specific targets for immunotherapy of gastrointestinal stromal tumors(GISTs)and using MAGE-1 and NY-ESO-1 mRNA levels as auxiliary parameters for risk classification of GISTs.METHODS:The expression of MAGE-1 and NY-ESO-1 mRNAs was detected by reverse transcription-polymerase chain reaction(RTPCR)in 30 GIST tissue specimens.The cor-relation of MAGE-1 and NY-ESO-1 mRNA expression with pathological ... |
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| Keywords: | MAGE-1 NY-ESO-1 |
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