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| 姜黄素致HepG-2细胞的凋亡作用及对bcl-2/bax表达的影响 | |
| 引用本文： | 贺庆芝,曾怀才,于伟霞,李国庆,易岚.姜黄素致HepG-2细胞的凋亡作用及对bcl-2/bax表达的影响[J].实用医学杂志,2010,26(3). |
| 作者姓名： | 贺庆芝 曾怀才 于伟霞 李国庆 易岚 |
| 作者单位： | 1. 南华大学药学与生命科学院生物科学系,湖南省衡阳市,421001 2. 公共卫生学院劳动卫生与环境卫生学教研室 3. 长清中学,济南市,250308 |
| 基金项目： | 湖南省卫生厅资助课题 |
| 摘 要： | 目的:研究姜黄素致人肝癌HepG-2细胞的凋亡作用及bax与bcl-2 mRNA表达的变化,为姜黄素的临床应用提供实验依据.方法:当HepG-2细胞处于对数生长期时,分别加入不同浓度(0、2.5、5、10、20、30 mg/L)的姜黄素处理48 h,MTT试验检测细胞的抑制率;流式细胞仪检测细胞凋亡率;实时荧光定量PCR检测bax和bcl-2mRNA的含量.结果:随着姜黄素浓度的增高,其对HepG-2细胞的抑制作用逐渐增强.当姜黄素浓度为30mg/L时,其抑制率达到(58.23±2.56)%,IC50=25.44 mg/L,而细胞的凋亡率由对照组的(1.1±0.3)%上升到(38.4±1.5)%;bax和bcl-2分别是对照组的(5.00±0.55)倍和(0.28±0.05)倍.结论:姜黄素能抑制HepG-2细胞的生长,诱导细胞凋亡,它的分子机制可能是上调bax基因和下调bcl-2基因的表达. |
| 关 键 词： | 肝肿瘤 姜黄素 凋亡 |
Effects of curcumin on apoptosis and the expressions of bcl-2/bax in HepG-2 cells |
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| HE Qing-zhi,ZENG Hum-cai,YU Wei-xia,LI Guo-qing,YI Lan.Effects of curcumin on apoptosis and the expressions of bcl-2/bax in HepG-2 cells[J].The Journal of Practical Medicine,2010,26(3). | |
| Authors: | HE Qing-zhi ZENG Hum-cai YU Wei-xia LI Guo-qing YI Lan |
| Institution: | HE Qing-zhi,ZENG Huai-cai,YU Wei-xia,LI Guo-qing,YI Lan.School of Biological Science,Pharmacy , Bioscience College,Nanhua University,Hengyang 421001,China |
| Abstract: | Objective To explore the effects of curcumin on apoptosis and expressions of bax and bcl-2 in hepatic cancer HepG-2 cells.Methods HepG-2 cells which were in logarithmic growth state were incubated with different concentrations(0,2.5,5,10,20,30 mg /L) of curcumin for 48 hours.The cells growth inhibitory rate was measured by MTT,apoptosis was observed by flow cytometry analysis,and the expressions of bax and bcl-2 were detected by real time PCR.Results The inhibitory effects of curcumin on HepG-2 cells were i... |
| Keywords: | bax bcl-2 |
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