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| 应用亲和层析分离纯化人小脑乙酰胆碱酯酶的初步研究 | |
| 引用本文： | 张兴梅,石玉生,张翰. 应用亲和层析分离纯化人小脑乙酰胆碱酯酶的初步研究[J]. 南方医科大学学报, 1999, 19(2): 165-167 |
| 作者姓名： | 张兴梅 石玉生 张翰 |
| 作者单位： | 1. 第一军医大学,生化教研室,广州,510515 2. 第一军医大学,南方医院放疗科,广州,510515 3. 军事医学科学院毒物药物研究所,北京,100850 |
| 摘 要： | 目的分离纯化人小脑乙酰胆碱酯酶（ＡＣｈＥ）。材料和方法采用ＣｏｎＡ和民Ｂ１０亲和层析法纯化人小脑ＡＣｈＥ。以纯化的人小脑ＡＣｈＥ为免疫原，免疫６只ＢＡＬＢ／ｃ小鼠制备小鼠抗人小脑ＡＣｈＥ抗血清，并检测其与电鳐ＡＣｈＥ和人红细胞膜ＡＣｈＥ。Ｅ的交叉免疫反应性。结果ＳＤＳ－ＰＡＧＥ还原状态下呈一条主带，相对分子质量为６７０００，比活性为３７３Ｕ／ｍｇ。结论免疫亲和层析法操作简单，可快速纯化人小脑ＡＣｈＥ。 |
| 关 键 词： | 免疫亲和层析 小脑 乙酰胆碱酯酶 交叉免疫反应性 |
A preliminary study of the purification acetylcholinesterase from human cerebellum by immuno-affinity chromatography |
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| Zhang Xingmei,Shi Yusheng,Zhang Han. A preliminary study of the purification acetylcholinesterase from human cerebellum by immuno-affinity chromatography[J]. Journal of Southern Medical University, 1999, 19(2): 165-167 | |
| Authors: | Zhang Xingmei Shi Yusheng Zhang Han |
| Affiliation: | Zhang Xingmei; Shi Yusheng; Zhang Han(Department of Biochemistry. Department of Radiotherapy of Nanfang Hospital. First Military Medical University. Guangzhou.5 1 05 15 |
| Abstract: | Objective To purify acetylcholinesterase (AChE) from human cerebellum. Materials and Methods By using ConA andB10, Sepharose affinity chromatographies to purify AChE. To prepare the mouse anti-human brain AChE antiserum. six BALB/cmice were irnmurtized with the puritied human cerebellum AChE. The cross-immunoreactivities of the antiserum with TorpedoAChE and human RBC membrane AChE were also detected. Results SDS-PAGE of the purified AChE which had a specific activityof, 373 U/mg under reduced condition showed one main band corresponding to a molecular weight of 67 000. Conclusion lmmunoaffinity chromatography is very simple and can be used to purify AChE from human cerebellum quickly. |
| Keywords: | immuno-affinity chromatography. cerebellum f acetylcholinesterase cross-immunoreactivity |
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