| 运用差异显示筛查冠心病血瘀证相关基因 |
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| 引用本文： | 王阶,姜燕,杨保林. 运用差异显示筛查冠心病血瘀证相关基因[J]. 中国中医药信息杂志, 2005, 12(2): 84-85 |
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| 作者姓名： | 王阶 姜燕 杨保林 |
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| 作者单位： | 1. 中国中医研究院西苑医院,北京,100091
2. 天津天士力集团,天津,300402 |
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| 基金项目： | 国家中医药管理局科研基金(02-03JP47)，创新工程基金(CX-00-02)项目，博士后基金(033227) |
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| 摘 要： | 目的 筛查冠心病血瘀证相关基因。方法 从临床入手,选择符合冠心病诊断和血瘀证辨证标准的患者,设计对照组,择用3个锚定4个随机引物,进行外周血mRNA差异显示,运用差异显示扫描系统扫描电泳胶图。结果 48条泳道中相同引物不同分组间出现了100余条差异条带。结论 mRNA差异显示是筛查冠心病血瘀证病证结合相关基因差异条带的可行方法之一。
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| 关 键 词： | 差异显示 血瘀证 冠心病 筛查 差异条带 |
| 文章编号： | 1005-5304(2005)02-0084-02 |
| 修稿时间： | 2004-05-09 |
Screening the Correlation Genes of Blood Stasis Syndrome in Coronary Heart Disease with mRNA Differential Display |
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| WANG Jie,JIANG Yan,YANG Bao-lin. Screening the Correlation Genes of Blood Stasis Syndrome in Coronary Heart Disease with mRNA Differential Display[J]. Chinese Journal of Information on Traditional Chinese Medicine, 2005, 12(2): 84-85 |
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| Authors: | WANG Jie JIANG Yan YANG Bao-lin |
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| Abstract: | Objective Identify the correlation genes of blood stasis syndrome in coronary heart disease. Method According to clinical diagnosis, the patients with blood stasis and coronary heart disease are selected as volunteers, and the normal population is set up as control group. In this experiment, we use 3 anchoring and 4 random primers to amplify mRNA samples from periphery blood of volunteers and the differential display results are demonstrated by gel imaging system. Result More than 100 differential bands can be observed in total 48 lanes responsible for different groups. Conclusion mRNA differential display is an applicable method for screening correlation genes of blood stasis syndrome in coronary heart disease. |
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| Keywords: | differential display blood stasis syndrome coronary heart disease screening differential band |
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