| 健骨二仙丸提取物对大鼠体外培养成骨细胞PKC和Bag-1的影响 |
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| 引用本文： | 陈小砖,李全,卿茂盛,萧劲夫. 健骨二仙丸提取物对大鼠体外培养成骨细胞PKC和Bag-1的影响[J]. 中国中医骨伤科杂志, 2010, 0(2): 6-8 |
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| 作者姓名： | 陈小砖 李全 卿茂盛 萧劲夫 |
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| 作者单位： | 深圳市中医院; |
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| 摘 要： | 目的:探讨健骨二仙丸提取物对大鼠体外培养成骨细胞的作用机理。方法:采用新生大鼠颅骨进行成骨细胞分离,并在含10%的胎牛血清DMEM中进行体外培养,分别加入高、低剂量的健骨二仙丸提取物并与高、低剂量Premarin组、阴性组、Tamoxifen对照组、Tamoxifen阻断对照组进行对照,用Western免疫印迹检测蛋白激酶C、抗凋亡蛋白Bag-1表达。结果:健骨二仙丸提取物能明显提高体外成骨细胞PKC的表达量和抗凋亡蛋白Bag-1的表达量。结论:健骨二仙丸提取物可促进成骨细胞增殖并明显提高PKC和抗凋亡蛋白Bag-1的表达量是其防治骨质疏松的机理之一。
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| 关 键 词： | 健骨二仙丸提取物 成骨细胞 体外培养 大鼠 蛋白激酶C 抗凋亡蛋白Bag-1 |
Effect of Jiangu Erxian Pill Extract on PKC and Bag-1 in Rat Osteoblast in Vitro |
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| CHEN Xiaozhuan LI Quan QING Maosheng XIAO Jingfu. Effect of Jiangu Erxian Pill Extract on PKC and Bag-1 in Rat Osteoblast in Vitro[J]. Chinese Journal of Traditional Medical Traumatology & Orthopeics, 2010, 0(2): 6-8 |
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| Authors: | CHEN Xiaozhuan LI Quan QING Maosheng XIAO Jingfu |
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| Affiliation: | CHEN Xiaozhuan1 LI Quan1 QING Maosheng1 XIAO Jingfu11Department of Special Chinese Medicine,Shenzhen Hospital of Traditional Chinese Medicine,Shenzhen 518033,China |
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| Abstract: | Objective:To explore the effect of Jiangu Erxian Pill Extract(JEPE) on rat osteoblast cells in vitro.Methods:The osteoblasts were separated from the skull of new-born rats,cultured in DMEM containing 10% bovine serum,and then divided into JEPE group(high dosage,low dosage),Premarin group(high dosage,low dosage),blank group,Tamoxifen group,and Tamoxifen inhibiting group.Western blotting was applied to measure the expression of PKC and anti-apoptotic protein BAG-1.Results:The expression of PKC and BAG-1 was s... |
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| Keywords: | Jiangu Erxian Pill Extract Osteoblast Culture in vitro Rat Protein kinase C Anti-apoptotic protein BAG-1 |
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