大豆皂甙对胰岛素下的平滑肌细胞脂质过氧化反应的影响

目的 :观察大豆皂甙对高浓度胰岛素下兔血管平滑肌细胞脂质过氧化反应的影响。方法 :采用兔主动脉平滑肌细胞培养 ,细胞随机分为正常对照组、胰岛素组及不同浓度大豆皂甙组 ,加药后继续培养 72 h,计数后测定细胞内过氧化脂质 (LPO)及超氧化物歧化酶 (SOD)含量。结果 :单纯高浓度胰岛素升高细胞内 LPO水平 ,降低细胞内 SOD活力 ,大豆皂甙与胰岛素共同孵育后 ,升高SOD活力 ;大豆皂甙低于 2 0 mg/L时抑制 LPO的产生 ,高于或等于 50 mg/L时促进 LPO的合成。结论 :高浓度胰岛素通过升高平滑肌细胞内 LPO促进动脉粥样硬化的形成 ,一定浓度的大豆皂甙抑制胰岛素所致脂质过氧化反应 ,对动脉粥样硬化具有防治作用

Effects of soyasaponin on cultured smooth muscle cell lipid peroxidation induced by insulin

Objective:The effect of different doses of total soyasaponin (TS) on lipid peroxidation were studied in rabbit vascular smooth muscle cell (SMC) induced by high dose of insulin.Methods:SMC culture technique was applied and the concentration of intracellular lipid peroxide (LPO) and activity of superoxide dismutase (SOD) were detected.Results:High dose of insulin increased the production of LPO and decreased the activity of SOD.But TS enhanced the activity of SOD and decreased the production of LPO in low dose (≤20 mg/L ) but increased the production of LPO in high dose (≥50 mg/L).Conclusion:Hyperinsulinmia might be an important factor in atherosclerosis.TS protects SMC from perxidation and atherogenesis.