| 蛋白酶活化受体2对大鼠肝星状细胞分泌MCP-1的诱导作用 |
| |
| 引用本文： | 顾小红,张云东.蛋白酶活化受体2对大鼠肝星状细胞分泌MCP-1的诱导作用[J].重庆医学,2009,38(3). |
| |
| 作者姓名： | 顾小红 张云东 |
| |
| 作者单位： | 1. 第三军医大学大坪医院野战外科研究所高干科,重庆,400042
2. 第三军医大学大坪医院野战外科研究所神经外科,重庆,400042 |
| |
| 基金项目： | 第三军医大学中青年基金 |
| |
| 摘 要： | 目的 探讨蛋白酶活化受体2(PAR2)对大鼠肝星状细胞(HSC)分泌单核细胞趋化蛋白-1(MCP-1)的调控作用.方法 选用培养7d的SD大鼠HSC并接种于培养板各孔内,分别用不同浓度PAR2激动剂、活性肽、反活性肽和抑制剂刺激,ELISA法检测培养上清MCP-1水平.结果 PAR2激动剂和活性肽可引起HSC MCP-1释放增加,抑制剂可抑制HSC促MCP-1的释放作用,其反活性肽不引起MCP-1释放增加.结论 在大鼠肝纤维化炎症反应中,PAR2可促进HSC分泌MCP-1,促进炎症反应,其反活性肽和抑制剂可能具有抗炎作用.
|
| 关 键 词： | 蛋白酶活化受体 肝星状细胞 单核细胞趋化蛋白-1 |
Protease-activated receptor 2 challenges rat hepatic stellate cell to produce MCP-1 |
| |
| GU Xiao-hong,ZHANG Yun-dong.Protease-activated receptor 2 challenges rat hepatic stellate cell to produce MCP-1[J].Chongqing Medical Journal,2009,38(3). |
| |
| Authors: | GU Xiao-hong ZHANG Yun-dong |
| |
| Institution: | GU Xiao-hong1,ZHANG Yun-dong2(1.Department of Senior Cadres Ward,2.Department of Neurologic Surgery,Daping Hospitall,Third Military Medical University,Chongqing 400042,China) |
| |
| Abstract: | Objective To investigate the actions of protease-activated receptor 2(PAR2)on the secretion of monocyte chemoattractant protein 1(MCP-1)from rat hepatic stellate cells(HSC).Methods HSC after 7d isolated and cultured from SD rats livers were transferred in a 24-well culture plate.The challenge was performed by addition of various concentrations of PAR2 agonist peptides SLIGRL and its reverse peptides LRGILS,trypsin and trypsin inhibitor named soybean trypsin inhibitor(SBTI)into each wel1 respectively.After 2... |
| |
| Keywords: | protease-activated receptor 1 hepatic stellate cells monocyte chemoattractant protein 1 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
