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| 胶体金标记单克隆抗体测定单增李斯特菌试剂盒制备 | |
| 引用本文： | 罗雁非,贾芙蓉,丁旭,方玲,郑岚,何成彦.胶体金标记单克隆抗体测定单增李斯特菌试剂盒制备[J].中国实验诊断学,2009,13(9):1231-1234. |
| 作者姓名： | 罗雁非 贾芙蓉 丁旭 方玲 郑岚 何成彦 |
| 作者单位： | 1. 吉林出入境检验检疫局,吉林,长春130000 2. 长春解放军第208医院,检验科 3. 吉林大学中日联谊医院,检验科 |
| 基金项目： | 国家质检总局计划项目 |
| 摘 要： | 目的制备单增李斯特菌54001、54002型的单克隆抗体（McAb），制备胶体金标记单增李斯特菌抗体免疫层析检测试剂板。方法以纯化单增李斯特菌为抗原，免疫Balb／c小鼠，制备单克隆抗体，鉴定其特性，建立并验证胶体金标记单增李斯特菌抗体免疫层析试剂板的检测方法。结果筛选出2株单抗杂交瘤细胞株F5、F9，免疫球蛋白亚类均为IgM。通过选用柠檬酸钠法制备胶体金，测定其最适结合蛋白浓度为28g/ml，制备胶体金标记单增李斯特菌抗体免疫层析检测板。结论为产单核李斯特菌提供了一个方便、快捷、切实有效的检测技术。 |
| 关 键 词： | 单增李斯特菌 单克隆抗体 胶体金 免疫层析 |
Gold immunochromato-graphy assay coupled with monoclonal antibodies against Listeria monocytogenes for detection of Listeria monocytogenes |
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| Institution: | LUO Yan-fei, JIA Fu-rong , Ding xu, et al. ( Jilin Entry-Exit Inspection and Quarantine Bureau, Changchun 130000, China) |
| Abstract: | Objective To prepare monoclonal antibodies against Listeria monocytogenes and to creat a colloidal gold immunochromato graphy assay fordetecting Listeria monocytogenes. Methods Purified Listeria monocytogenes was used as antigen to immune Balb/c mice. Monoclonal antibodies against Listeria monocytogenes were prepared by normal hybridoma technology . Colloidal gold was coupled with the antibodies against Listeria monocytogenes and Listeria monocytogenes was detected by immunochromatography assay in this study.Results Colloidal gold was prepared by eitrate-natrium method and the best compatible protein concentration was measured as 25 ug/ml, therefore, gold immunochromato graphy assay for detection was founded. Conclusion The detection method is aconvenient, quick, effective technology for Lizteria monocytogenes. |
| Keywords: | listeria monocytogenes monoclonal antibody colloidal gold immunochromatography |
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