昂丹司琼局部用药抗伤害作用的实验研究

目的研究局部昂丹司琼应用的抗伤害作用。方法(1)局部昂丹司琼的抗伤害效应,实验小鼠随机分为昂丹司琼组(2、8、32mg/ml),利多卡因组(20mg/ml)和DMSO组(n=8)。每组小鼠均局部接受2min不同浓度的药物浸润处理。在随后的180min内分别在小鼠尾部药物处理过的部位和未处理部位观察其热痛阈甩尾潜伏期的变化;(2)药物处理时间与昂丹司琼抗伤害效应间的关系,随机分为5组(n=8),各组小鼠尾部远端分别暴露于32mg/ml昂丹司琼药液中30s、1、2、5、10、30min后测定甩尾潜伏期。结果(1)在药物局部处理过的小鼠尾部,利多卡因和昂丹司琼产生了明显的抗伤害反应作用,昂丹司琼的这种作用是浓度相关性的。(2)在一定的时间范围内,局部昂丹司琼的抗伤害效应的强度与局部暴露于药液的时间相关。结论局部应用昂丹司琼起到了浓度相关的、有效的、抗伤害反应的效应。

Experimental study on antinociceptive effect of ondansetron in local use

Objective To investigate the antinociceptive effect of ondansetron in local use. Methods (1)Antinociceptive actions of topical ondansetron in mice. Mice were randomly divided into ondansetron group(2mg/ml,8mg/ml,32mg/ml),lidocaine group(20mg/ml) and DMSO group(n=8).Groups of mice received a 2 min topical exposure to different concentrations of drugs.Tail-flick latencies for each animal were measured from regions of the tail exposed to the compound or not across a 180 min time period.(2)Effect of exposure time on the antinociceptive actions of topical ondansetron in mice. Groups of mice received 0.5, 1.0, 2.0 ,5.0,10 min topical exposure to a fixed concentration of ondansetron(32 mg/ml). Tail flick latencies were obtained 30 min following topical exposure.Results (1)Lidocaine administration produced antinociceptive effect .Ondansetron administration produced concentration-dependent antinociception.(2)The antinociceptive effect of topical ondansetron was dependent on the exposure time.Conclusion Topical administration of ondansetron elicits concentration-dependent antinociception.