三七皂甙对急性脑梗塞患者血浆和脑脊液MCP-1、ET含量的影响

研究三七皂甙对急性脑梗塞患者血浆和脑脊液单核细胞趋化因子（MCP 1）和内皮素（ET）水平在不同时段变化的影响。方法：用酶联免疫法和放射免疫法测定MCP 1和ET在不同时间血浆和脑脊液含量的变化情况，及应用三七皂甙治疗后的影响。结果：脑梗塞患者血浆MCP 1和ET的水平明显增加，三七皂甙组各时间段MCP 1和ET的含量与对照组比较有显著性差异（P<0.05）,MCP 1在第3天达到峰值，第5天开始下降。ET在第3～5天达峰值，之后逐渐下降，第10天的含量仍高于第1天。脑梗塞48?h后患者脑脊液MCP 1和ET的含量亦明显升高。应用三七皂甙后血浆MCP 1和ET的水平均显著低于对照组（P<0.01）。结论： 脑梗塞后血浆和脑脊液MCP 1和ET水平明显增高（P<0.05）；MCP 1和ET的变化有一定相关性（r=0.852）。三七皂甙对脑梗塞后血浆和脑脊液MCP 1和ET的表达有部分抑制作用，这可能是三七皂甙减轻脑梗塞后缺血缺氧的分子机制之一。

Role of saponins on MCP-1 and ET in serum and cerebrospinal fluid of patients with acute brain infarction

Objective:To investigate the concention changes of the monocyte chemoattractant protein-1(MCP-1) and the endothelia(ET) in serum and cerebrospinal fluid(csf) of patients with acute brain infarction treated by saponins.Methods: The level of MCP-1and ET in different times in serum and cerebrospinal were determined by enzyme-linked immunoadsordent(ELLISA) and radioreceptor assay(RRA). CSF samples were obtained on the first day of the onset and at 48 hours later during the lumbar puncture procedure.Results: Both the serum levels of MCP-1 and ET in different times were notably higher in ischemic stroke than healthy man(P<0.05).The level of MCP-1 reached the peak on the day 3 and then decreased on the day 5-10,and that of ET,too.The levels of MCP-1 andET significantly increased 48 hours after the onset of brain infarction,but they significantly decreased after the treatment of saponins(P<0.05). Conclusions: Our results suggest that MCP-1 and ET may play roles in the inflammatory reaction during the early phase of ischemic stroke;the increasing of MCP-1 can stimulate the secretion and expression of ET;saponins can partly resist the secretion and expression of ET and MCP-1,which may be one of the mechanism for decreasing the danger of cerebroinfarction.