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| K-ras基因转染鼠肺纤维细胞粘附分子的表达及临床意义 | |
| 引用本文： | 张其刚,宿杰,尚文军,关永信.K-ras基因转染鼠肺纤维细胞粘附分子的表达及临床意义[J].中国医科大学学报,2003,32(5):394-395,441. |
| 作者姓名： | 张其刚 宿杰 尚文军 关永信 |
| 作者单位： | 1. 中国医科大学附属第一医院胸外科,辽宁,沈阳,110001 2. 沈阳市第七人民医院胸外科 3. 营口市经济技术开发区人民医院 |
| 摘 要： | 目的：探讨细胞粘附分子在肿瘤细胞浸润转移中的意义。方法：利用免疫荧光标记及聚丙烯酰胺凝胶电泳和westem blott观察K-ras基因转染的鼠肺(KTRL)纤维细胞及正常鼠肺(NRL)纤维细胞钙粘素-链蛋白复合体分子的表达。结果：KTRL纤维细胞粘附分子表达明显异常，肌蛋白分布方式发生改变，粘附能力明显下降。结论：KTRL纤维细胞作为肿瘤细胞的早期阶段，其粘附能力的改变，可能是后期形成肿瘤细胞后发生浸润转移的主要原因。 |
| 关 键 词： | K-ras转染鼠肺纤维细胞 粘附分子 浸润 转移 |
| 文章编号： | 0258-4646(2003)05-0394-02 |
Expression and Clinical Significance of Adhesive Molecule in Lung Fibrocyte of K-ras Transformed Rat |
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| ZHANG Qi-gang ,SU Jie ,SHANG Wen-jun ,GUAN Yong-xin.Expression and Clinical Significance of Adhesive Molecule in Lung Fibrocyte of K-ras Transformed Rat[J].Journal of China Medical University,2003,32(5):394-395,441. | |
| Authors: | ZHANG Qi-gang SU Jie SHANG Wen-jun GUAN Yong-xin |
| Institution: | ZHANG Qi-gang 1,SU Jie 2,SHANG Wen-jun 1,GUAN Yong-xin 3 |
| Abstract: | Objective: Our aim was to study the function of adhesive molecule in the process of tumor cell invasion. Methods: We studied lung fibrocytes in K-ras transformed rats and normal rats. The expression of cadherin-catenin complex was observed with immunofluorescence labeling technique, polyacrylamide gel electrophoresis, and western blot. Results: Obviously abnormalities was found in the expression of adhesive molecule of lung fibrocytes in K-ras transformed rats. The distribution of actin changed with significance decrease of adhesion. Conclusion: The change of cell adhesion in early stage of tumor may be a major factor for cell invasion and metastasis in advanced stage. |
| Keywords: | K-ras transformed rat lung fibrocyte adhesive molecule invasion metastasis |
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