柱前衍生化法测定丽江产玛咖中18种氨基酸含量 |
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引用本文: | 高萌,李明仙,李宇赤,王婉丽,李光. 柱前衍生化法测定丽江产玛咖中18种氨基酸含量[J]. 中国科学美容, 2014, 0(6): 33-36,43 |
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作者姓名: | 高萌 李明仙 李宇赤 王婉丽 李光 |
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作者单位: | [1]西双版纳傣族自治州医院中药房,云南景洪666100 [2]西双版纳傣族自治州职业技术学院,云南景洪666100 [3]中国医学科学院药用植物研究所云南分所,云南景洪666100v |
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基金项目: | 中央级公益性科研院所基本科研业务专项(YZYN-11-03);中国医学科学院北京协和医学院青年科研基金项目(XHYN-01,3332013081) |
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摘 要: | 目的测定丽江产玛咖中必需氨基酸的含量。方法以2,4-二硝基氟苯为衍生试剂,与丽江产玛咖中18种氨基酸柱前衍生,用Waters600高效液相色谱仪,菲罗门公司Symmetry C18色谱柱,以含1%N,N-二甲基甲酰胺的0.05mol/L醋酸钠-醋酸缓冲液(pH=6.4)及乙腈:水(1:1)进行梯度洗脱,检测波长为360nm,柱温为35℃,进样量为20μL。结果18种氨基酸的线性回归方程r值为0.9995~1.0000(n=5),平均回收率为99.54%-101.84%,RSD值为0.38%~1.82%,n=6。结论本法快速、简便,结果准确。
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关 键 词: | 柱前衍生化 玛咖 氨基酸 2,4-二硝基氟苯 高效液相色谱法 |
Contents of 18 amino acids in MACA using pre-column derivatization method |
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Affiliation: | GAO Meng LI Mingxian LI Yuchi WANG Wanli LI Guan( 1.Dispensary of Traditional Chinese Medicine,Dai Autonomous Prefecture of Xishuangbanna Hospital, Jinghong 666100,China; 2.Xishuangbanna Vocational and Technical InstituteJinghong 666100,China; 3.Yunnan Branch, Institute of Medicinal Plant,Chinese Academy of Medical Sciences,Peking Union Medical CoUege,Jinghong 666100,China) |
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Abstract: | Objective To analyze the contents of 17 amino acids in MACA using pre-column derivatization method. Methods The amino acids in MACA were pretreated with 2,4-dinitro-fluorobenzene and their derivatives were analyzed on Waters 600 HPLC using Symmetry Cls column. Gradienteluents consisted of sodium acetate solution(pH=6.4) with 1% N,N-dimethylformamide, acetonitrile and water(1 : 1). The Detection wavelength was 360nm and column temperature was 35℃ . Results The calibration curves for 18 amino acidshad good linearity(r=0.9995 - 1,n=5). The mean recoveries of 18 amino acids were 99.54%-101.84% with RSD 0.38%-1.82%(n=6). Conclusion The method is quick, simple for the content analysis of the amino acids in the injections. |
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Keywords: | Pre-column derivatization MACA Amino acids DNFB HPLC |
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