维生素C对百草枯中毒小鼠肺组织细胞凋亡及Bcl-2/Bax蛋白表达的影响

目的　探讨维生素C对百草枯中毒后小鼠肺组织的细胞凋亡及Bcl 2、Bax蛋白表达影响。方法　复制小鼠百草枯中毒模型 ,对其用 10 0mg/kg、 2 0 0mg/kg、 4 0 0mg kg三种剂量的维生素C进行干预。采用原位末段标记检测 (TUNEL法 )和免疫组化检测技术观察中毒后和经维生素C干预后肺组织细胞凋亡数及Bcl 2、Bax蛋白的表达。结果　中毒组肺组织细胞凋亡数明显增加 (P <0 0 5 ) ,应用 10 0mg/kg剂量的维生素C干预时有较明显的抗凋亡作用 (P <0 0 5 ) ,而 4 0 0mg/kg剂量又有促使细胞凋亡的作用 (P >0 0 5 )。中毒组Bcl 2和Bax蛋白表达水平均低于维生素C干预组 (分别P <0 0 1和P <0 0 5 ) ,经维生素C干预后Bcl 2和Bax蛋白表达明显上调。结论　维生素C能促进百草枯中毒后肺组织Bcl 2、Bax蛋白表达 ,且不同剂量作用时分别产生抑制细胞凋亡和促进细胞凋亡的作用

Effects of Vitamin C on apoptosis and expression of Bcl-2/Bax protein in lung tissue of rats with paraquat poisoning

Objective To study the effects of vitamin C on apoptosis and expression of Bcl-2 and Bax protein in lung tissue in rats with paraquat(PQ)poisoning.Methods By using situ end labeling(TUNEL)and immunnocytochemistry techniques,apoptosis and expression of Bcl-2/Bax protein were assessed in lung tissues in rats with PQ poisoning and in rats intervened by vitamin C(100mg/kg,200mg/kg,400mg/kg)before PQ poisoning.Results Apoptosis in lung tissue in rats with PQ poisoning significantly increased.A dose of 100mg/kg vitamin C before PQ poisoning inhibited apoptosis(P<0.05),while 400mg/kg vitamin before poisoning accelerated apoptosis(P>0.05).Expression of Bcl-2 and Bax in poisoning group were significantly lower than those in rats interrened by vitamin C group(P<0.01,P<0.05 respectively).Bcl-2 and Bax were up-regulated after intervened by vitamin C.Conclusion Vitamin C can facilitate Bcl-2 and Bax expression in lung tissue in rats with PQ poisoning,and under the action of different dose,it plays the role of and facilitation apoptosis.