| LC-MS/MS法测定人血浆中氯吡格雷的浓度及其药动学研究 |
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| 引用本文： | 季宏建,;周小华,;邹建军,;都述虎,;何振伟. LC-MS/MS法测定人血浆中氯吡格雷的浓度及其药动学研究[J]. 中国药房, 2009, 0(8): 584-586 |
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| 作者姓名： | 季宏建, 周小华, 邹建军, 都述虎, 何振伟 |
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| 作者单位： | [1]南京医科大学药学院,南京市210029; [2]南京医科大学附属南京市第一医院,南京市210006; [3]南通市第一人民医院药剂科,南通市226001 |
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| 摘 要： | 目的:建立以高效液相色谱串联质谱电喷雾检测(LC-MS/MS)法测定人血浆中氯吡格雷浓度的方法,并研究其在健康人体内的药动学。方法:以噻氯匹定为内标,血浆样品经液-液萃取后,采用汉邦-C18柱进行分离,通过三重四极杆串联质谱仪,以多反应监测(MRM)方式进行测定。结果:氯吡格雷血药浓度在10～10000pg.mL-1范围内线性关系良好(r=0.9992);平均相对回收率在98.7%～100.8%之间,日内、日间RSD均≤13.01%。结论:本方法快速、简便、准确、灵敏,适用于氯吡格雷的人体药动学研究。
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| 关 键 词： | 高效液相色谱串联质谱电喷雾检测法 氯吡格雷 血药浓度 药动学 |
Determination of Clopidogrel Concentration in Human Plasma by LC- MS/MS and Study on Its Pharmacokinetics |
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| Affiliation: | JI Hong-jian, ZHOU Xiao-hua, DU Shu-hu, ZOU Jian-jun, HE Zhen-wei(School of Pharmacy, Nanjing Medical University, Nanjing 210029, China;Nanjing First Hospital Affiliated to Nanjing Medical University, Nanjing 210006, China;Dept. of Pharmacy, Nantong First People＇s Hospital, Nantong 226001, China) |
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| Abstract: | OBJECTIVE： To establish an LC MS/MS method for the determination of plasma clopidogrel concentration and to study its pharmacokinetics in healthy volunteers. METHODS： Taking ticlopidine as internal standard, after liquidliquid extraction, the plasma sample was separated on hanbon - （218 column and detected by multiple reaction monitoring （MRM） on a triple quadrupoles tandem mass spectrometer. RESULTS： The linear range of clopidogrel was 10-10 000 pg · mL ^-1 （r = 0.999 2）, average relative recovery at 98.7%- 100.8%, and RSD of inter day and intra--day at no more than 13.01%. CONCLUSION： The method developed in the study was proved to be rapid, simple, accurate and sensitive, and suitable for the pharmacokinetic study of clopidogrel in healthy volunteer. |
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| Keywords: | LC- MS/MS Clopidogrel Plasma concentration Pharmacokinetics |
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