决明子生品及炮制品的HPLC指纹特征研究

目的:研究决明子生品及炮制品的高效液相色谱(HPLC)指纹特征,为建立决明子药材的HPLC指纹图谱提供依据。方法:样品用1.5mol.L-1盐酸溶液加热水解,用氯仿回流提取,采用梯度洗脱技术进行HPLC分离分析。用聚类分析方法处理色谱指纹信息,比较色谱以大黄酚为参照色谱峰的18个特征峰的信息、单波长的全部指纹信息及220～550nm全波长的二维指纹图谱的信息。结果:决明子生品及炮制品中有数种化学成分的含量高于大黄酚的含量。决明子生品与炮制品的色谱指纹特征比较接近,但有差别。结论:所得指纹图谱中决明子水解液的脂溶性成分分离良好,大黄酚、大黄素、大黄素甲醚3个纯色谱峰得到确认。采用220～550nm全波长二维指纹图谱的指纹信息较采用单波长全部色谱指纹信息及特征峰指纹信息的聚类结果更加可靠。

HPLC Fingerprint Profiles of Crude and Processed Seeds of Semen Cassiae

OBJECTIVE： To study the HPLC fingerprint profiles of crude and processed Semen Cassiae so as to provide basis for the establishment of chromatographic fingerprint of Semen Cassiae.METHODS： The samples of Semen Cassiae were hydrolyzed in the 1.5mol·L^-1 hydrochloride acid and then reflux extracted with chloroform. The HPLC analysis was run in gradient elution.The whole information of single- wavelength chromatographic fingerprint, two-dimension information data at full wavelength 220 nm～550 nm and the data of 18 fingerprint peaks compared with chrysophanol were treated by cluster analysis.RESULTS： It was observed that the contents of several chemical components were higher in the crude or processed Semen Cassiae than in chrysophanol. There were differences between the crude and the processed Semen Cassiae in HPLC fingerprint profiles though they were of good similarity.CONCLUSION： The HPLC fingerprints of liposoluble constituents were obtained with good resolution under the experimental chromatographic system. Three pure chromatographic peaks of chrysophanol, emodin and physcion have been identified. More reliable cluster result can be obtained from two- dimension information data at full wavelength 220nm～550nm than from the whole information of single - wavelength chromatographic fingerprint or from the information of chromatographic fingerprint of characteristic peaks.