| HPLC法测定盐酸米诺环素缓释片主药的含量 |
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| 引用本文： | 齐靖,;蒋海松,;吴琳华. HPLC法测定盐酸米诺环素缓释片主药的含量[J]. 中国药房, 2009, 0(10): 781-782 |
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| 作者姓名： | 齐靖, 蒋海松, 吴琳华 |
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| 作者单位： | [1]哈尔滨医科大学附属第二医院药学部,哈尔滨市150086; [2]哈尔滨神龙中药药物研究所,哈尔滨市150030 |
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| 摘 要： | 目的:建立以高效液相色谱法测定盐酸米诺环素缓释片主药含量的方法。方法:色谱柱为Thermo ODS-C_(18),流动相为0.2 mol·L~(-1)草酸铵-0.01 mol·L~(-1)乙二胺四乙酸二钠-二甲基甲酰胺-四氢呋喃(600:180:120:80),检测波长为280 nm,进样量为20μL,流速为1.5 mL·min~(-1)。结果:米诺环素检测浓度的线性范围为0.05～0.5 mg·mL~(-1)(r=0.999 8);平均回收率为99.11%,RSD=0.5%。结论:本方法准确可靠、灵敏度高、重现性好,可用于该制剂的含量测定。
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| 关 键 词： | 盐酸米诺环素 高效液相色谱法 缓释片 含量测定 |
Determination of Main Components in Minocycline Hydrochloride Sustained - release Tablets by HPLC |
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| Affiliation: | QI Jing, WU Lin-hua, JIANG Hai-song(1.Dept. of Pharmacy, The Second Affiliated Hospital, Harbin Medical University, Harbin 150086, China;2.Shen Long Institute of Traditional Chinese Medicine, Harbin 150030, China) |
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| Abstract: | OBJECTIVE： To establish an HPLC method for determining the content of main components in minocycline hydrochloride sustained- release tablets. METHODS： The sample was separated on ODS- C18 column with the mobile phase consisted of 0.2 mol·L^-1 ammonium oxalate - 0.01 mol·L^-1 edetate disodium- dimethylformamide- tetrahydrofuran （600 ： 180 ： 120 ： 80） at a flow rate of 1.5 mL · min ^-1. The detection wavelength was set at 280 nm and the sample volume was 20 μL. RESULTS： The linear range of minocycline was 0.05-0.5 mg ·mL ^-1 （r = 0. 999 8） and the average recovery was 99.11%, RSD ： 0.5%. CONCLUSIONS： This method was proved to be accurate and reliable, sensitive and reproducible, and it can be used for determining the content of this tablets. |
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| Keywords: | Minocycline hydrochloride HPLC Sustained-release tablets Content determination |
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