RP-HPLC法测定导便栓中醋酸氯己定的含量

目的:建立以反相高效液相色谱法测定导便栓中醋酸氯己定含量的方法。方法:色谱柱为Diamond C_(18),流动相为乙腈-水-三乙胺(30:70:0.5,用磷酸调pH至3.0),检测波长为260 nm,流速为1.0 mL·min~(-1),进样量为20μL,柱温为30℃。结果:醋酸氯己定检测浓度的线性范围为1.092～13.10μg·mL~(-1)(r=0.999 9);平均回收率为98.29%(RSD=2.16%,n=9)。结论:本方法操作简便、准确、灵敏,可用于该制剂的含量测定。

Content Determination of Chlorhexidine Acetate in Daobian Suppository by RP- HPLC

OBJECTIVE： To establish a RP- HPLC method for the determination of the content of chlorhexidine acetate in daobian suppository. METHODS： The determination was performed on Diamonsil C18 column, The mobile phase consisted of acetonitrile- water- triethylamine （30 ： 70 ： 0.5, with pH adjusted to 3.0 by phosphoric acid） at a flow rate of 1.0 mL · min^-1, The detective wavelength was 260 nm, the sample size was 20μ L and the column temperature was maintained at 30 ℃. RESULTS： The linear range of Chlorhexidine Acetate was 1.092-13.10μg · mL^-1 （r = 0.999 9） and its average recovery was 98.29% （RSD = 2.16%, n = 9） . CONCLUSIONS： The method is simple and accurate, and it can be used for the determination of the content of chlorhexidine acetate in daobian suppository.