白芍总苷对慢性非细菌性前列腺炎大鼠CD4+T淋巴细胞及IL-6的影响研究

目的:研究白芍总苷(TGP)对慢性非细菌性前列腺炎(CAP)大鼠前列腺组织中CD4+T淋巴细胞及白细胞介素-6 (IL-6)的影响及其对CAP的作用和机制。方法:将36只SD大鼠随机分为CAP对照组、TGP低剂量组、TGP高剂量组,每组12只。给药后观察大鼠前列腺组织的病理形态学变化;免疫组化法检测CD4+T淋巴细胞及IL-6在各组大鼠前列腺中的表达。结果:CAP对照组大鼠前列腺组织呈明显炎症状态,TGP低剂量组腺体结构改善,有少量炎症细胞浸润,TGP高剂量组炎症状态明显消退,只有极少量炎症细胞散在分布;与CAP对照组比较,TGP低、高剂量组CD4+T淋巴细胞及IL-6的表达均显著降低(P<0.05)。结论:TGP对CAP具有治疗作用,其机制可能是抑制前列腺组织中CD4+T淋巴细胞等炎症细胞的浸润,降低IL-6等促炎症细胞因子的过高表达。

Effects of Total Glucosides of Paeony on the Expression of CD4 ＋ T Lymphocytes and IL - 6 in Rats with Chronic Abacterial Prostatitis

OBJECTIVE： To investigate the effects of total glucosides of paeony （TGP） on the expressions of CD4 ＋ T lymphocytes and IL - 6 in prostatic tissues of rats with chronic abacterial prostatitis （CAP） and to study its action mechanism on CAP. METHODS： 36 SD rats were randomly divided into three groups： CAP control group （ n= 12 rats）, low dose CAP ＋ TGP group（ n = 12） and high dose CAP ＋ TGP group（ n - 12） .The expressions of CD4 ＋ T lympholeukocytes and IL - 6 in prostatic tissues of rats were detected by immunohistochemistry after medication were investigated, and the changes in histopathoiogy of prostatic tissues. RESULTS： Significant inflammatory condition was noted in CAP control group; improvement of glandular structure and infiltration of small number of inflammatory cells were noted for low dose TGP group. In high dose TGP group, inflammation was abated markedly and there was diffused distribution of a few number of inflammatory cells. As compared with CAP group, the expressions of CD4＋ T lymphocytes and IL- 6 were down - regulated significantly in both the high dose and low dose TGP groups （P〈0.05） .CONCLUSION： The therapeutic effect TGP on CAP may be achieved through inhibitions on the infiltration of CD4 ＋ T lymphocytes as well as on the overexpression of pro - inflammatory cytokines such as IL 6.