异丙酚对油酸性急性肺损伤模型大鼠肺组织细胞凋亡的影响 |
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引用本文: | 沈颜红,;张建新. 异丙酚对油酸性急性肺损伤模型大鼠肺组织细胞凋亡的影响[J]. 中国药房, 2009, 0(1): 30-32 |
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作者姓名: | 沈颜红, 张建新 |
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作者单位: | [1]河北省胸科医院,石家庄市050041; [2]河北省疾病预防控制中心,石家庄市050021 |
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基金项目: | 河北省科学技术研究与发展指导计划项目(20042761465) |
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摘 要: | 目的:探讨静脉麻醉药异丙酚对油酸性急性肺损伤(ALI)模型大鼠肺组织细胞凋亡的影响。方法:取40只SD大鼠随机分成正常对照组、ALI组及异丙酚低、中、高剂量(4、8、16mg·kg-1·h-1)组。除正常对照组不输注油酸外,其余4组大鼠均于颈静脉输注油酸,之后各组输注相应药物4h腹主动脉放血处死,留取肺组织,光镜观察肺病理变化,流式细胞仪检测肺组织细胞凋亡率及凋亡相关蛋白Fas/FasL、Bax/Bcl-2的表达情况。结果:与正常对照组比较,ALI组肺组织细胞凋亡率显著升高(P<0.05);Bax、Fas和FasL蛋白表达均显著增强(P<0.05);Bcl-2蛋白表达显著降低(P<0.05)。与ALI组比较,异丙酚中、高剂量组肺组织细胞凋亡率、Bax、Fas和FasL蛋白表达均显著降低(P<0.05),Bcl-2蛋白表达均显著增强(P<0.05),但低剂量组上述指标变化均不显著。结论:异丙酚可能通过调控油酸性ALI时肺组织Bax/Bcl-2、Fas/FasL系统的活化而显著抑制肺组织细胞凋亡,减轻肺损伤,从而发挥一定的保护作用。
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关 键 词: | 异丙酚 急性肺损伤 大鼠 细胞凋亡 油酸 |
Effect of Propofoi on Apoptosis in Lung Tissue of Rats with Oleic Acidinduced Acute Lung Injury |
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Affiliation: | SHEN Yan-hong,ZHANG Jian-xin(Hebei Chest Hospital, Shijiazhuang 050041, China;2.Hebei Center for Disease Control and Prevention, Shijiazhuang 050021, China) |
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Abstract: | OBJECTIVE: To investigate the effect of propofol on apoptosis in lung tissue of oleic acidinduced acute lung injury (ALI) rats. METHODS: Forty male SD rats were randomly divided into normal control group, ALI group, low, medium and high-dose (4, 8, 16 mg· kg^-1· h^-1) propofol groups. Except for the normal control group, the other 4 groups were infused with oleic acid through jugular vein followed by the infusing of the corresponding drugs for 4 h. Then the rats were sacrificed by exsanguination. Lung tissue was immediately sampled for observation of the pathologic change under light microscope and detection of apoptosis and the expression of Fas/FasL,Bax/Bcl-2 in lung tissue by FCM. RESULTS: In ALI group compared with the normal control group, the apoptosis rates in lung tissue cells was markedly increased (P 〈 0.05); the expression of Bax, Fas and FasL in lung tissue were significantly upregulated (P 〈 0.05) while the expression of Bcl- 2 was significantly down- regulated (P〈 0.05) . Compared with ALI group, medium and high dose propofol groups significantly down- regulated the apoptosis rates and the expressions of Bax, Fas and FasL (P 〈 0.05), significantly upregulated Bcl- 2 expression (P〈0.05) . However, there were not significant change in all above indexes in low-dose propofol group. CONCLUSIONS: It could be concluded that propofol has a protective function on ALI induced by oleic acid through inhibiting the apoptosis in lung tissue by regulating the activation of Bax/Bcl-2 and Fas/FasL systems. |
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Keywords: | Propofol Acute lung injury Rat Apoptosis Oleic acid |
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