| HPLC法测定健脾颗粒中橙皮苷的含量 |
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| 引用本文： | 林清,;吴雪梅,;马晓鹂,;符小英.HPLC法测定健脾颗粒中橙皮苷的含量[J].中国药房,2009(9):698-699. |
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| 作者姓名： | 林清 ;吴雪梅 ;马晓鹂 ;符小英 |
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| 作者单位： | [1]广东医学院附属医院药学部,湛江市524001; [2]广东恒诚制药有限公司,湛江市524022 |
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| 摘 要： | 目的:建立以高效液相色谱法测定健脾颗粒中橙皮苷含量的方法。方法:色谱柱为Kromasil C18(200mm×4.6mm,5μm),流动相为乙腈-0.3%磷酸溶液(20∶80),检测波长为283nm,流速为1.0mL·min-1。结果:橙皮苷的检测浓度在9.92～99.75μg·mL-1范围内与峰面积积分值呈良好线性关系(r=0.9999);平均回收率为99.1%,RSD=0.2%(n=6)。结论:本方法简便、准确、重现性好,可用于健脾颗粒的质量控制。
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| 关 键 词： | 健脾颗粒 高效液相色谱法 橙皮苷 含量测定 |
Determination of Hesperidin in Jianpi Granules by HPLC |
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| Institution: | LIN Qing, WU Xue-mei, MA Xiao-li, FU Xiao-ying(Dept. of Pharmacy, The Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China)(Guangdong Hengcheng Pharmaceutical Co. Ltd., Zhanjiang 524022, China) |
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| Abstract: | OBJECTIVE： To establish HPLC method for the determination of the conent of hesperidin in Jianpi granules （granules for invigorating the spleen）. METHODS： Kromasil C18（200 mm × 4.6 mm, 5μm） was used as the chromatographic column, and the mobile phase consisted of acetonitrile - 0.3% phosphoric acid solution （20 ： 80） at a flow rate of 1.0 mL ·min^-1 under a detection wavelength of 283 nm. RESULTS： The linear range of hesperidin was 9.92-- 99.75μg·mL^-l（r = 0.999 9） with an average recovery rate of 99.1% （RSD = 0.2%, n = 6） . CONCLUSION： The method is simple, accurate, reproducible, and it can be used for the quality control of Jianpi granules. |
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| Keywords: | Jianpi granules HPLC Hesperidin Content determination |
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