红花对2型糖尿病大鼠肾脏氧化应激反应的影响

目的:研究红花对2型糖尿病大鼠肾脏氧化应激反应的影响。方法:采用单侧肾切除加高脂高糖和小剂量链脲佐菌素的方法建立2型糖尿病大鼠模型。分为正常对照、模型和红花低、高剂量组。治疗12周后测定各组生化指标、尿白蛋白排泄率(UAER)和肾皮质超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)活性以及丙二醛(MDA)含量,检测肾皮质P22phox mRNA、P47phox mRNA表达水平。结果:与模型组比较,红花各剂量组血糖水平无显著性差异;血肌酐、尿素氮、UAER水平极显著下降;SOD、GSH-PX活性显著上升;MDA含量极显著下降;P22phox mRNA、P47phox mRNA表达水平显著降低。结论:红花能减轻糖尿病大鼠肾皮质氧化应激反应,有保护肾脏和延缓糖尿病肾病发生的作用。

Effects of Carthamus Tinctorius on Oxidative Stress of Kidney in Rats with Diabetic Nephropathy

OBJECTIVE： To study the effects of Carthamus tinctorius on oxidative stress of kidney in rats with type 2 diabetes mellitus. METHODS： Type 2 diabetes mellitus rat model was developed successfully by unilateral nephreetomy and giving high lipid and high glucose diet and low dose streptozotocin （STZ）. Then the model rats were randomly divided into four groups： control group, model group, low- dose and high-dose Carthamus tinctorius groups. After treatment for 12 weeks, biochemical indicators, urinary albumin excretion rate （UAER）, the activities of superoxide dismutase （SOD） and Glutathione peroxidase （GSH- PX） and the content of malonaldehyde （MDA） in the renal tissue, and the mRNA gene expressions of both P22phox and P47phox in renal cortex were measured. RESULTS： Compare with model group, Cartharnus tinctorius -treated groups （high- dose and low- dose） showed no significant difference in blood glucose level, but much significant decrease in levels of serum creatinine, urea nitrogen and UARE, significant increase in SOD and GSH- PX activities, much significant decrease in MDA content and significant downregulation of mRNA expressions of both P22phox and P47phox. CONCLUSION： Carthamus tinctorius -treatment can mitigate the oxidative stress of renal cortex to play protective effect on kidney and delay the development of diabetic nephropathy.