紫外-可见分光光度法测定右旋糖酐铁制剂中铁的含量

目的:建立以紫外-可见分光光度法测定右旋糖酐铁制剂中铁含量的方法。方法:在酸性条件下,加热右旋糖酐铁溶液使Fe~(3+)游离后,用盐酸羟胺还原成Fe~(2+),再与邻二氮菲形成红色络合物,以紫外-可见分光光度法在510 nm波长处测定其含量。结果:Fe~(2+)检测浓度的线性范围为0.5～4.0μg·mL~(-1)(r=0.999 9);右旋糖酐铁片及其注射液的平均回收率分别为99.8% (RSD=0.52%)、99.7%(RSD=0.44%)。结论:本方法操作简便、结果准确,可用于该类制剂的含量测定。

Content Determination of Fe in Iron Dextran Preparations by UV- VIS

OBJECTIVE： To establish the ultraviolet visible spectrophotometry（UV - VIS） for the determination of Fe content in iron dextran preparations. METHODS： Under the acidic condition, Fe^3＋ was dissociated from iron dextran solution by heating, then Fe^3＋ was deoxidized into Fe^2 ＋ by hydroxylamine hydrochloride, and red Fe^2＋ -orthophenanthroline complex was formed through combination with o - phenanthroline. The content of the Fe^2 ＋ - ortbopbenanthroline complex was determined by UVVIS at a wavelength of 510 nm. RESULTS： The linear range of Fe^2 ＋ was 0.5-4.0 μg · mL ^-1 （r = 0.999 9）. The average recoveries of two preparations （tablets and injection） of iron dextran were 99.8% （RSD = 0.52%） and 99.7% （RSD = 0.44% ）, respectively. CONCLUSION： The UV-VIS method is simple, accurate in results and applicable for the determination of iron dextran preparations.