D101型大孔吸附树脂纯化油茶皂苷的工艺研究

目的:研究D101型大孔吸附树脂纯化油茶粕中油茶皂苷的工艺。方法:以油茶皂苷的含量为指标,考察D101型大孔吸附树脂的上样量、吸附容量和水溶性杂质、油茶皂苷洗脱的乙醇浓度及体积。结果:纯化工艺:D101型大孔吸附树脂的上样量为3 BV,吸附容量为566 mg·g~(-1)生药量,使用3BV蒸馏水和1 BV 20%乙醇洗脱水溶性杂质,3 BV85%乙醇洗脱油茶皂苷。结论: D101型大孔吸附树脂可较好地纯化油茶皂苷,该工艺简单,成本低,适用于工业化大生产。

Purification Technology of Camellia Saponin Using DIO1 Macroporous Adsorption Resin

OBJECTIVE： To study the purification technology of camellia saponin using D101 macroporous adsorption resin. METHODS： The loading volume of sample and the adsorption quantity of D101 macroporous adsorption resin and the concentration and volume of alcohol in eluting water soluble foreign substances and total cemallia saponins were investigated taking the content of total cemallia saponins as index. RESULTS： The loading volume of sample of D101 macroporous adsorption resin was 3 fold that of resin D101 （or 3 BV, resin bed volume） and its adsorption quantity was 566 mg · g^-1（crude drug） ;3 BV distilled water and 1 BV 20% ethanol were used for the elution of the water-soluble foreign substances, and 3BV of 85% ethanol was used for the elution of total cemallia saponins. CONCLUSION： D101 macroporous resin has good purification efficacy for camellia saponins. The purification process is simple with low cost, and it is applicable for large - scale industrialized production.