瘦素对乳腺癌MCF-7细胞增殖的影响及调控机制的研究

目的:研究瘦素对人乳腺癌MCF-7细胞增殖的影响及对雌、孕激素受体和VEGF的mRNA表达水平的影响,进而揭示瘦素对乳腺癌MCF-7细胞系作用的分子生物学机制。方法:体外培养人乳腺癌MCF-7细胞系,分为对照组、瘦素组和雌激素组。给药后用MTT法测570nm分光光度值。实时荧光定量PCR法观察各组雌、孕激素受体及VEGF的mRNA表达水平。结果:与对照组相比,瘦素可以呈时间和剂量依赖的方式促进MCF-7细胞增殖,并上调细胞中雌、孕激素受体及VEGF的mRNA表达水平,P<0.05。结论:瘦素有促进乳腺癌细胞增殖的作用,该作用的发挥可能与其上调雌、孕激素受体与VEGF的表达有关。为乳腺癌的内分泌治疗提供了新的理论依据。

Effects of leptin on breast cancer MCF-7 cells proliferation in vitro by regulation of estrogen receptor and VEGF

OBJECTIVE:To observe the proliferation of MCF-7 cells and the mRNA expression of estrogen receptor(ER),progesterone receptor(PGR)and VEGF after giving different concentrations of leptin or estrogen to MCF-7 cells,and to reveal the molecular biological mechanism of the effect of leptin on ER positive breast cancer cells.METHODS:After the culture of MCF-7 cells for 72 h in vitro,the cells were treated with leptin,estrogen or vehicle as control.After the medication,the 570 nm OD values of cells were read by MTT and the mRNA expressions of ER,PGR,and VEGF were observed with real-time PCR.RESULTS:Compared with the control group,leptin promoted the proliferation of MCF-7 cells in a dose and time dependent manner,and increased the mRNA expressions of ER,PGR,and VEGF,significantly(P<0.05).CONCLUSIONS:Leptin promotes breast cancer cells proliferation,and probably up-regulates the mRNA expressions of ER,PGR,and especially VEGF.This finding may provide a new theoretical basis for breast cancer endocrinotherapy.