糖尿病大鼠视网膜早期病变组织中生长因子作用的实验研究

目的研究糖尿病大鼠视网膜早期病变组织中生长因子的作用机制.方法腹腔注射四氧嘧啶,制备糖尿病模型,成模后3个月活体取跟球,制备视网膜病理切片及视网膜单分散细胞悬液.应用ABC法和酶联免疫吸附法对视网膜组织中碱性成纤维细胞生长因子(bFGF)和血管内皮细胞生长因子(VEGF)进行定性和定量分析.结果Wistar大鼠糖尿病视网膜组织中bFGF和VEGF含量分别为1 3.50±1.80ng/ml,14.25±0.70ng/ml,同对照组3.06±1.40ng/ml,4.50±0.85ng/ml比较有显著差异(P<0.01).实验组bFGF和VEGF在视网膜胞浆内呈阳性表达.病理切片观察,视网膜内层细胞及微血管出现阳性病理改变.结论bFGF和VEGF在糖尿病大鼠视网膜早期病变组织中起重要的介导作用,并反映了视网膜病变的严重程度.

Experimental study on growth factor in the early phase of retinopathy of diabetic rats

Objective: To investigate the effects of growth factors in the early phase of retinopathy tissue of diabetic rats. Methods: 30 Wistar rats were divided into control and experimental groups. Twenty rats models were made by alloxan abdomen injection, ten rats were used as healthy control groups. After three months we extracted eyes in alive. Analytic methods included light and electron microscopes, immunohistochemical ABC and ELISA. Results: There was a significant difference between the two groups statistically (P<0.01). The levels of bFGF and VEGF in experimental group were l3.50 ( 1.80ng/ml and 14.25 ( 0.70ng/ml, respectively and the immunohistochemical stain for bFGF and VEGF revealed positive responses. Obvious pathologic changes could be seen in the inner retina of experimental group. Conclusion: The outcome implied that bFGF and VEGF might play an important role in the early course of diabetic retinopathy.