| 牛蒡子中牛蒡甙及其甙元反相高效液相色谱法测定(英文) |
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| 引用本文： | 孙文基,沙振方,高海.牛蒡子中牛蒡甙及其甙元反相高效液相色谱法测定(英文)[J].药学学报,1992,27(7):549-551. |
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| 作者姓名： | 孙文基 沙振方 高海 |
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| 作者单位： | 陕西省药品检验所，陕西省药品检验所，陕西省药品检验所 西安 710061，西安 710061，西安 710061 |
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| 摘 要： | 从牛蒡子中分离出牛蒡甙,用酶解方法得到甙元。对牛蒡子中牛蒡甙及其甙元用HPLC法测定了含量。使用碳-18反相色谱柱,甲醇-水(1:1)为流动相,UV270mm作为检测波长。样品只需用甲醇浸泡,超声波浴提取20min,取上清液进样分析。方法简便,适于中药牛蒡子的定量分析。
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| 关 键 词： | 牛蒡子 牛蒂甙 牛蒡甙元 高效液相色谱法 |
| 收稿时间： | 1991-07-24 |
Determination of arctiin and arctigenin in Fructus Arctii by reverse-phase HPLC. |
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| WJ Sun,ZF Sha,H Gao.Determination of arctiin and arctigenin in Fructus Arctii by reverse-phase HPLC.[J].Acta Pharmaceutica Sinica,1992,27(7):549-551. |
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| Authors: | WJ Sun ZF Sha H Gao |
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| Institution: | Shaanxi Provincial Institute for Drug Control, Xian. |
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| Abstract: | The fruits of Arctium lappa L. is an often-used herbal drug in traditional Chinese medicine for the treatment of common cold caused by wind and heat. This drug contained many constituents, principally arctiin, with arctigenin in smaller amount. In this work, arctiin has been isolated from the fruits of Arctium lappa, and then enzymolyzed into arctigenin. The obtained arctiin and arctigenin were characterized and then used as standards for their determination in the crude drug by HPLC. The method is simple, rapid and accurate. |
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| Keywords: | Arctiin Arctigenin HPLC Arctium lappa L |
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