载脂蛋白E基因多态性与芪参益气滴丸治疗气虚血瘀型冠心病的关系

目的探讨载脂蛋白E(ApoE)基因多态性与芪参益气滴丸治疗气虚血瘀型冠心病疗效的关系。方法符合诊断标准的冠心病患者49例,服用芪参益气滴丸1个月。提取白细胞DNA,扩增ApoE基因第4外显子,测序检测多态性位点。结果发现2个有义突变位点,第2888位突变与气短、神疲乏力的治疗效果有关;第2903位突变对胸痛、自汗的治疗效果有显著影响。结论ApoE基因多态性与芪参益气滴丸治疗气虚血瘀型冠心病证候的疗效有相关性。

Relation of Apolipoprotein E Gene Polymorphism and Qishen Yiqi Droplet in Treatment of Coronary Heart Disease of Qi Deficiency with Blood Stasis Syndrome

Objective To study the relation between apolipoprotein E(ApoE) gene polymorphism and the effect of Qishen Yiqi Droplet(Droplet mainly containing Huangqi and Danshen for Reinforcing Qi) for qi deficiency with blood stasis syndrome of coronary heart disease.Methods The 49 patients conformed to the diagnosis standard of coronary heart disease were treated with Qishen Yiqi Droplet for one month.The white blood cell DNA was extracted from the blood sample and the 4th exon of ApoE gene was tested for finding its polymorphism locus with sequencing.Results Two significant mutable sites were noticed:the mutation of the 2888th site was remarkably related to the therapeutic effect of shortness of breath and general lassitude while that of the 2903rd site to the thera- peutic effect of chest pain and spontaneous sweating.Conclusion ApoE gene polymorphism was related to the effect of qi deficiency with blood stasis syndrome of coronary heart disease treated by,Qishen Yiqi Droplet.