Prokinetic and laxative activities of Lepidium sativum seed extract with species and tissue selective gut stimulatory actions |
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Authors: | Najeeb-Ur-Rehman Mehmood Malik Hassan Alkharfy Khalid M Gilani Anwarul-Hassan |
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Institution: | a Natural Product Research Division, Department of Biological and Biomedical Sciences, The Aga Khan University Medical College, Karachi 74800, Pakistan b Department of Pharmacology, Faculty of Pharmacy, University of Karachi 75270, Pakistan c Department of Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia |
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Abstract: | Aim of the studyTo provide ethnopharmacological basis for the medicinal use of Lepidium sativum seeds in indigestion and constipation.Materials and methodsThe in vivo studies were conducted in mice, while isolated tissues of mouse, guinea-pig and rabbit were suspended in tissue bath to measure isotonic contractions.ResultsThe aqueous-methanolic extract of Lepidium sativum seeds (Ls.Cr) at 30 and 100 mg/kg showed atropine-sensitive prokinetic and laxative activities in mice, which were partially sensitive to atropine. In isolated gut preparations of mouse and guinea-pig, Ls.Cr (0.1-1 mg/mL) caused a concentration-dependent stimulatory effects both in jejunum and ileum, which was blocked in the presence of atropine. In rabbit jejunum, the stimulant effect of Ls.Cr remained unchanged in the presence of atropine, pyrilamine or SB203186, while in rabbit ileum, the stimulatory effect was partially blocked by atropine. The Ls.Cr was more efficacious in gut preparations of rabbit than in guinea-pig or mouse. The phytochemical analysis of the plant extract detected alkaloids, saponins and anthraquinones as plant constituents.ConclusionThis study showed the prokinetic and laxative effects of Lepidium sativum in mice, which were partially mediated through a cholinergic pathway. The in vitro spasmodic effect of the plant extract mediated through a similar mechanism with species and tissue-selectivity, provides a rationale for the medicinal use of the seeds of Lepidium sativum in indigestion and constipation, and suggests studying the plant extracts on more than one species to get the wider picture. |
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Keywords: | Lepidium sativum Cruciferae Prokinetic Laxative Cholinergic Ileum Jejunum Species-specific Rodents |
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