Protection against 1-methyl-4-phenylpyridinium ion (MPP+)-induced apoptosis by water extract of ginseng (Panax ginseng C.A. Meyer) in SH-SY5Y cells |
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Authors: | Hu Shengquan Han Renwen Mak Shinghung Han Yifan |
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Institution: | a Department of Applied Biology and Chemical Technology, Institute of Modern Chinese Medicine, The Hong Kong Polytechnic University, Hong Kong, China b State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, China |
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Abstract: | Ethnopharmacological relevanceThe present study investigates the protective effects of water extract of ginseng (Panax ginseng C.A. Meyer) against 1-methyl-4-phenylpyridinium ion (MPP+)-induced cytotoxicity in SH-SY5Y human neuroblastoma cells and explores the underlying mechanisms. The approach may be used for screening therapeutic agents for degenerative disorders such as Parkinson's disease.Materials and methodsSH-SY5Y human neuroblastoma cells were used to analyze the protective effects of water extract of ginseng (WEG) against multiple parameters such as MPP+-induced viability, oxidative injury, expression of Bax, Bcl-2, cytochrome c and cleaved caspase-3.ResultsWEG exerted inhibitory effect on cell death, overproduction of ROS, elevated Bax/Bcl-2 ratio, release of cytochrome c and activation of caspase-3 expression in MPP+-treated SH-SY5Y cells.ConclusionsWEG exhibited significant protective effects against MPP+-induced cytotoxicity in SH-SY5Y cells possibly through the suppression of ROS generation and the inhibition of mitochondria-dependent apoptotic pathway. |
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Keywords: | Ginseng (Panax ginseng C A Meyer) Water extract of ginseng (WEG) MPP+ Reactive oxygen species (ROS) Mitochondrial dysfunction Parkinson's disease (PD) |
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