| HPLC法测定中药退黄外洗液中栀子苷、柚皮苷的含量 |
| |
| 引用本文： | 林冬杰.HPLC法测定中药退黄外洗液中栀子苷、柚皮苷的含量[J].中国药师,2010,13(4):523-524. |
| |
| 作者姓名： | 林冬杰 |
| |
| 作者单位： | 梧州食品药品检验所,广西梧州,543002 |
| |
| 摘 要： | 目的：建立HPLC法同时测定中药退黄外洗液中栀子苷、柚皮苷含量。方法：色谱柱：AgilentSB—C18（250mm×4．6mm，5μm），流动相：以乙腈-水梯度洗脱，流速1．0ml·min-1，检测波长，238nm和283nm。结果：栀子苷在107—1075ng范围内线性关系良好r=0．9999，平均回收率为99．3％，RSD=O．30％（n=6）；柚皮苷在119～1190ng范围内线性关系良好r=0．9999，平均回收率为99．2％，RSD=0．24％（n=6）。结论：操作简便，准确可靠，可作为中药退黄外洗液的质量控制。
|
| 关 键 词： | 中药退黄外洗液 HPLC 栀子苷 柚皮苷 |
HPLC Determination of Geniposide and Naringin in Zhongyao Tuihuangwai Lotions |
| |
| Lin Dongjie.HPLC Determination of Geniposide and Naringin in Zhongyao Tuihuangwai Lotions[J].China Pharmacist,2010,13(4):523-524. |
| |
| Authors: | Lin Dongjie |
| |
| Institution: | Lin Dongjie (Wuzhou Institute for Food and Drug Control, Guangxi Wuzhou 543002 ,China) |
| |
| Abstract: | Objective： To establish an HPLC method for determination of geniposide and naringin in zhongyao tuihuangwai Lo- tions. Method： A Agilent SB-CIs Column （250 mm ×4.6 mm,5μLm） was used,the mobile phase was Acetonitrile-water with gradient elution, the detection wavelength were 238 nm and 283 nm, the flow rate was 1.0 ml-min-l. Result： The linear ranges of geniposide and naringin were 107-1 075 ng,r = 0. 999 9 and 119-1 190 ng, r = 0. 999 9, the average recoveries （n = 6 ） were 99.3% （RSD = 0.30% ） and 99.2% （RSD 0.24% ） ,respectively. Conclusion： This method is simple,and accurate. It can be used for quality control of Zhongyaotuihuangwai Lotions. |
| |
| Keywords: | HPLC |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
