人胚胎脑细胞在大鼠脑梗死灶内的分化和整合

目的 探讨人胚胎脑细胞在脑梗死灶内的分化、整合状况和神经细胞移植治疗脑梗死的可能性。方法 将正常人胚胎脑细胞和胶质细胞混合培养后，移植到免疫抑制的11只肾性高血压大鼠的大脑皮质梗死灶内，并与未移植的5只脑梗死大鼠作对照。8周后取大鼠脑组织做免疫组化检查。结果移植了人胚胎脑细胞存活的lO只大鼠中，6只有移植物生长。移植物内有新生血管，细胞有分层排列趋势。免疫组化染色证实，移植物内有细胞分化，并存在大量神经元和星形胶质细胞。神经元的树突和轴突汇集成束，整合到宿主脑内。结论 培养的人胚胎脑细胞能在免疫抑制大鼠的脑梗死灶内生长、分化和整合，提示神经细胞移植有望成为临床治疗脑梗死的突破性方法。

Differentiation and integration of cultured human fetal brain cells in infarct cavity of rat brain

Objective To observe the differentiation and integration of cultured human fetal brain cells in infarct cavity of rat brain and to explore the possibility for treatment of brain infarction with neural transplantation. Methods Cultured neurons and glial cells from human embryonic brain were transplanted to the infarct cavity in 11 immunodepressed stroke-prone renovascular hypertensive rats brains. Five sham transplanted rats served as controls. Immunohistochemistry was performed on sections from brains perfused in situ 8 weeks after transplantation. Results Obvious grafts were observed in 6 of 10 survived rats. There were blood vessels grown into the grafts. The morphology of the grafts showed a tendency of organized laminar character. Numerous neurons and astrocytes with long processes were observed. The axons and dendrites gathered and formed fiber bundles were protruded into the host brain. Conclusion These results suggest that cultured human fetal brain cells can survive and differentiate in infarct cavity and integrate with host brain in immunodepressed rats. Neural transplantation is a potential novel therapy for stroked patients.