| RT-PCR检测R2L1细胞中Trk受体家族基因的表达 |
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| 引用本文： | 明洪,张闻,李京昆,曾惠芳,黄秉仁. RT-PCR检测R2L1细胞中Trk受体家族基因的表达[J]. 昆明医学院学报, 2002, 23(1): 44-47 |
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| 作者姓名： | 明洪 张闻 李京昆 曾惠芳 黄秉仁 |
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| 作者单位： | 1. 昆明医学院生物教研室,昆明,650031
2. 中国协和医科大学基础医学院,北京,100005 |
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| 基金项目： | 昆明医学院骨干教师基金 |
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| 摘 要： | 在Trk受体家族基因的非同源区中分别设计对TrkA，TrkB和TrkC受体基因特异的引物，采用RT－PCR法检测大鼠小脑神经细胞系R2和转染并表达了神经营养因子低亲和力受体p75NTR的R2细胞系R2L1细胞中Trk受体家族基因的表达，琼脂糖凝胶电泳分析RT－PCR产物表明，TrkA和TrkB受体基因在R1和R2L1细胞中均有表达，而无TrkC受体基因的表达。测序结果进一步证实了RT－PCR的结果，表明本方法能准确地检测Trk受体家庭基因的表达。
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| 关 键 词： | Trk受本家族 RT－PCR p75NTR R2L1细胞 神经营养因子 基因表达 |
| 文章编号： | 1003-4706(2002)01-0044-04 |
| 修稿时间： | 2001-10-20 |
Detection of Trk Receptor Family in R2L1 Cell Line by RT-PCR |
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| MING Hong ),ZHANG Wen ),LI Jing-kun ),ZENG Hui-fang ),HUANG Bing-ren ). Detection of Trk Receptor Family in R2L1 Cell Line by RT-PCR[J]. Journal of Kunming Medical College, 2002, 23(1): 44-47 |
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| Authors: | MING Hong ) ZHANG Wen ) LI Jing-kun ) ZENG Hui-fang ) HUANG Bing-ren ) |
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| Affiliation: | MING Hong 1),ZHANG Wen 1),LI Jing-kun 1),ZENG Hui-fang 1),HUANG Bing-ren 2) |
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| Abstract: | We detected the expression of trkA, trkB and trkC genes in R2 and R2L1 cell lines derived from rat cerebellum by RT-PCR. R2L1 cell line was derived from R2 cells transfected with human p75NTR gene. Argarose gel and sequencing analysis showed the expression of trkA and trkB genes in both R2 and R2L1 cells, but the expression trkC was not detected. |
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| Keywords: | Trk receptor family RT-PCR p75NTR |
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