CD34、CD14、CD10、CD31和因子Ⅷ在人胚胎卵黄囊血岛、AGM区和肝造血细胞中的表达

目的研究CD34、CD31、CD14、CD10和凝血因子Ⅷ(FⅧ)在人胚胎发育第3～12周卵黄囊血岛、PAS／主动脉-生殖腺-中肾(AGM)区和肝造血细胞的表达。方法药物流产受精龄3～12周人胚胎共32例，石蜡包埋切片，苏木精一伊红和免疫组化染色，光镜观察。结果第3～4周人胚卵黄囊血岛由外周的血管内皮细胞和中心的造血细胞组成。血岛的血管内皮细胞和造血细胞均为CD10、CD14、CD31和FⅧ表达阳性。此外，血管内皮细胞还表达CD34，而造血细胞为CD34阴性。第32天，卵黄囊血岛的造血细胞已经迁移。在第4周，主动脉、中肾和肝造血灶内开始出现CD34、CD10、CD14、CD31和FⅧ阳性的造血细胞。到第7周，CD10、CD14阳性细胞数量达到最高。到第11～12周，上述各区域中多为无核的红细胞，CD34、CD10、CD14、CD31和FⅧ表达为阴性。第4～12周，胚体内所有血管内皮细胞均为CD34阳性。结论卵黄囊血岛造血细胞和内皮细胞共表达CD10、CD14、CD31和FⅧ，卵黄囊血管内皮细胞表达CD34，而造血细胞不表达CD34。背主动脉、中肾和肝的造血细胞主要在第4～7周表达CD34、CD10、CD14、CD31和FⅧ。抗CD34抗体可标记人胚胎3-12周各种血管的内皮细胞。

Expression of CD34, CD14, CD10, CD31 and factor Ⅷ on hematopoietic cells of yolk sac blood is land, AGM region and liver of human embryo and fetus

OBJECTIVES: To study the expression of CD34, CD31, CD14, CD10 and factor VIII on blood island of yolk sac (YS), PAS/aorta-germenmesonephros (AGM) region and hepatic hematopoietic foci. METHODS: Thirty-two cases of 3rd-12th weeks human embryo were obtained by mifisitone abortion. Paraffin embedded sections with H.E staining and immunohistochemistry reaction (SABC) were performed. RESULTS: YS blood island of 3rd-4th weeks of gestation was consisted of two types of cells. One was vascular endothelial cells located outside and the other hematopoietic cells inside the blood island. Both the two types of cells were CD10, CD14, CD31 and factor VIII positive. Hematopoietic cells were CD34 negative, and vascular endothelial cells were CD34 positive. On 32th days of gestation, the hematopoietic cells migrated out of YS. On 4th week of gestation, CD34, CD14, CD10, CD31 and factor VIII positive cells appeared in the aorta, mesonephros and hepatic hematopoietic foci. By the 7th week, the number of positive hematopoietic cells reached the peak. In 11th-12th weeks, most cells in these regions were matured red blood cells and were negative for all the antibodies mentioned above excepting for CD34. During 4th-12th weeks, all endothelial cells in embryo were CD34 positive. CONCLUSIONS: The hematopoietic cells and endothelial cells of YS blood island co-expressed CD10, CD14, CD31 and factor VIII. Endothelial cells were CD34 positive but hematopoietic cells were negative in YS blood island. The hematopoietic cells of aorta, mesonephros and hepatic hematopoietic foci expressed CD34, CD10, CD14 and factor VIII from 4th week to 7th week. Anti-CD34 antibody could label endothelial cells of every kinds vessels of embryo from 3rd to 12th weeks.