2型糖尿病患者白细胞介素-6和可溶性细胞黏附分子-1及血清超敏C-反应蛋白的血清水平分析

目的检测2型糖尿病患者尿微量白蛋白（UmALB）以及血清超敏C-反应蛋白（hs-CRP）、白细胞介素-6（IL-6）和可溶性细胞黏附分子-1（sICAM-1）的水平,探讨炎症对糖尿病肾病（DN）发生、发展的影响。方法根据尿微量白蛋白排泄率（UREA）将91例2型糖尿病患者分为正常蛋白尿组30例,早期DN组29例,蛋白尿组32例;选择91例同期健康体检者作为健康对照组。分析各组炎症因子血清表达水平的差异,UmALB用速率散射比浊法测定,hs-CRP用致敏乳胶增强免疫技术测定,IL-6、sICAM-1水平的测定用酶联免疫吸附（ELISA）方法。结果 2型糖尿病组患者血清中hs-CRP[（10.63±8.31）mg/L]、IL-6[（74.35±58.94）ng/L]以及sICAM-1水平[（113.93±42.26）μg/dl]明显高于健康对照组[分别为（2.89±3.27）mg/L、（29.32±17.09）ng/L、（27.15±13.97）μg/dl],差异均有统计学意义（P〈0.01）,不同UREA各组之间hs-CRP、IL-6以及sICAM-1的水平差异均有统计学意义（P〈0.05,P〈0.01）。UmALB与hs-CRP、IL-6、sICAM-1表达量呈明显的正相关（r值分别为0.8168、0.731 8和0.8567,P〈0.01）。结论炎症是DN肾病发生、发展中的重要影响因素。

Analysis on the serum levels of interleukin-6, soluble vascular cell adhesion molecular 1 and highly sensitive C-reactive protein in patients with type 2 diabetes

Objective To detect the levels of urinary microalbumin（UmALB）,serum highly sensitive C-reactive protein（hs-CRP）,interleukin-6（IL-6） and soluble vascular cell adhesion molecular 1（sICAM-1） in patients with type 2 diabetes（T2DM） and to explore the influence of inflammatory on development of diabetic nephropathy（DN）. Methods According to UmALB,91 cases with T2 DM were divided into normal urinary albumuria group（30 cases）,early DN group（29 cases） and proteinuria group（32 cases）,and 91 healthy residents served as controls. The levels of serum inflammatory factors among four groups were compared. UmALB was detected by ratenephelometry and hs-CRP was determined by sensitization latex enhanced immune technology. ELISA was used to detect IL-6 and sICAM-1. Results The serum levels of hs-CRP,IL-6 and sICAM-1 in T2 DM patients were（10.63±8.31） mg/L,（74.35±58.94） ng/L and（113.95±42.26） μg/dl,respectively,which were significantly higher than those [（2.89±3.27） mg/L,（29.32±17.09） ng/L and（27.15±13.97） μg/dl] in control group（P〈0.01）. There were significant differences of serum hs-CRP,IL-6 and sICAM-1 levels among different type 2 diabetic sub-groups（P〈0.05 or P〈0.01）. The serum levels of hs-CRP,IL-6 and sICAM-1 were correlated with UmALB level（r values were 0.816 8,0.731 8 and 0.856 7,P〈0.01）. Conclusion Inflammation is an important influencing factor on the development of DN.