Hybridization and cytoduction among yeast cells of the same mating type |
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Authors: | M V Repnevskaya T S Karpova S G Inge-Vechtomov |
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Institution: | (1) Department of Genetics, Leningrad State University, 199164 Leningrad, USSR |
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Abstract: | Summary Use of a selective system for cytoduction in Saccharomyces cerevisiae allowed us to monitor hybrid formation and to clone the haploid nuclei of cells which have participated in illegitimate matings: a × a, × . Our approach has made it possible to select nuclei with mating-type switches and mutations within the MAT locus. It was shown that matings in × crosses often proceed through nonheritable genetic changes located within chromosome III. We suggest that these non-heritable genetic changes are due to premutational lesions, expressed phenotypically as transient -matingtype. After a mating event these lesions are either repaired or converted to true mutations within the MAT locus. |
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Keywords: | Mating-type switching Cytoduction Saccharomyces cerevisiae |
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