Subgram daily supplementation with docosahexaenoic acid protects low-density lipoproteins from oxidation in healthy men |
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| Authors: | Catherine Calzada Romain Colas Nicolas Guillot Michel Guichardant Martine Laville Evelyne Véricel Michel Lagarde |
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| Institution: | 1. Université de Lyon, F-69622, Lyon, France;2. INSA de Lyon, RMND, F-69621, Villeurbanne, France;3. INSERM U870, IMBL, F-69621, Villeurbanne, France;4. INRA U1235, F-69921, Oullins, France;5. CRNH-RA, Hospices Civils de Lyon, F-69003, Lyon, France;1. Department of Food Science and Nutrition, Zhejiang University, Hangzhou, China;2. School of Medicine, Deakin University, Geelong, Australia;3. Department of Nutrition and Dietetics, Monash University, Melbourne, Australia;4. Institute for Physical Activity and Nutrition (IPAN), School of Exercise and Nutrition Sciences, Deakin University, Melbourne, Australia;5. Institute of Nutrition and Health, Qingdao University, Qingdao, China;1. Ecole Pratique des Hautes Etudes (EPHE) & Paris Research University, 75014 Paris, France;2. Université de Lorraine, Ecologie et Ecophysiologie Forestières (UMR INRA 1137), Faculté des Sciences et Technologies, BP70239, F54506 Vandoeuvre les Nancy, France;3. INRA, Ecologie et Ecophysiologie Forestières (UMR INRA 1137), F54280 Champenoux, France;4. Université Lyon 1, Laboratoire d’Écologie des Hydrosystèmes Naturels et Anthropisés (UMR 5023 CNRS), 69622 Villeurbanne, France;1. Epidemiology Unit, French Agency for Food, Environmental and Occupational Health & Safety (ANSES), 31 Avenue Tony Garnier, F-69364 Lyon, Cedex 07, France;2. Unité d''épidémiologie animale, UR346, INRA, F-63122 St Genès Champanelle, France;3. Direction générale de l''Alimentation, 251 rue de Vaugirard, F-75732 Paris Cedex 15, France;4. VetAgroSup Campus Vétérinaire, 1 avenue Bourgelat, F-69280 Marcy l''Etoile, France;5. Université de Lyon, F-69000 Lyon, France;6. Université Lyon 1, CNRS, UMR5558, Laboratoire de Biométrie et Biologie Evolutive, Bâtiment Mendel, 43 Bd du 11 novembre 1918, F-69622 Villeurbanne, France;1. Internal Medicine, Iketani Clinic, 3-7, Akigawa 1, Akiruno-shi, Tokyo 197-0804 Japan;2. Department of Cardiology, Tokyo Medical University Hachioji Medical Center, Japan;3. Second Department of Internal Medicine, Tokyo Medical University, Japan |
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| Abstract: | ObjectiveTo determine the effect of supplementation with increasing doses of docosahexaenoic acid (DHA), as the only n-3 polyunsaturated fatty acid (PUFA), on low-density lipoprotein (LDL) redox status and oxidizability.MethodsTwelve healthy men aged 53–65 years ingested consecutive doses of DHA (200, 400, 800 and 1600 mg/day), each dose for two weeks.ResultsThe proportions of DHA increased dose-dependently in LDL phospholipids and cholesteryl esters, even after two weeks of supplementation with 200 mg/day DHA. The daily intake of 200, 400 and 800 mg DHA resulted in increased alpha-tocopherol concentrations, decreased MDA concentrations, and a longer lag time for copper-induced LDL oxidation. Supplementation with 1600 mg/day DHA had no effect on the above parameters. In plasma, concentrations of 4-hydroxy-hexenal, specifically derived from the peroxidation of n-3 fatty acids, significanty increased after 800 and 1600 mg DHA, representing 0.01% of plasma n-3 PUFAs, while 4-hydroxy-nonenal concentrations, derived from the peroxidation of n-6 fatty acids, did not change.ConclusionOur results clearly show that an intake of 200–800 mg/day DHA may have protective and antioxidant effects on LDL and could represent optimal doses for cardiovascular disease prevention in a healthy population. |
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