电化学免疫发光分析法联合核酸检测定量法在ELISA HBsAg-/NAT+血液样本中的应用价值

目的 分析电化学免疫发光法(ECLIA)联合核酸检测(NAT)定量法在ELISA HBsAg-/NAT+血液样本检测中的应用价值.方法 选择血站采集的无偿献血者血液样本22843例,酶联免疫吸附法(ELISA)检测为乙型肝炎表面抗原(HBsAg)阴性的血液样本行NAT检测,先行混合检测,后行拆分检测.选取拆分检测为初检...

Application value of electro-chemiluminescence immunoassay combined with nucleic acid test quantitative method in ELISA HBsAg-/NAT+blood samples

Objective To analyze the application value of electro-chemiluminescence immunoassay(ECLIA)combined with nucleic acid test(NAT)quantitative method in enzyme linked immunosorbent assay(ELISA)for HBsAg-/NAT+blood samples.Methods A total of 22843 blood samples were collected from voluntary blood donors in blood stations.The blood samples with negative HBsAg detected by ELISA were detected by NAT,mixed detection first,and then split detection.The split detected were used for NAT quantitative test in blood samples with ELISA HBsAg-/NAT+,and the blood samples with positive NAT were further detected by ECLIA combined with NAT quantitative test.Results By ELISA detection,65 cases were HBsAg positive and 22778 cases were HBsAg negative in 22843 blood samples.A total of 22778 HBsAg negative blood samples by ELISA were further detected by NAT,after mixed detection,27 mixed sample pools were positive;after split detection,29 blood samples were positive of HBV-DNA.Twenty-nine blood samples of initial ELISA HBsAg-/NAT+were further detected by NAT quantitative,the positive rate was 65.52%(19/29),the viral load of HBV-DNA≥20 IU/mL in 5 cases and<20 IU/mL in 14 cases.Nineteen blood samples of ELISA HBsAg-/NAT+by ECLIA detection,HBsAg and HBeAg were all negative,HBsAb was positive in 3 cases(15.79%),HBeAb was positive in 7 cases(36.84%),HBcAb was positive in 15 cases(78.95%),serum pattern was all negative in 5 cases(26.32%),simple HBcAb was positive in 7 cases(36.84%).Nineteen blood samples of ELISA HBsAg-/NAT+tested by ECLIA combined with NAT quantitative test,4 cases was in the window period,15 cases was in the latent infection period.Conclusion There is a certain phenomenon of missing detection in the initial examination of blood samples in blood stations.NAT can quickly screen out HBV-DNA positive samples from a large number of negative ELISA samples.ECLIA combined with NAT quantitative test can help blood donors to identify their own HBV infection status.