钙库操纵性钙通道蛋白在环磷酰胺诱导的膀胱过度活动症大鼠模型膀胱组织中的表达

目的 制备膀胱过度活动症(overactive bladder,OAB)大鼠模型,探讨钙库操纵性钙通道蛋白在OAB大鼠膀胱组织中的表达.方法 30只健康雌性SD大鼠,随机分为模型组和对照组各15只.模型组单次腹腔注射环磷酰胺200 mg/kg制备OAB模型,对照组腹腔注射等量生理盐水.2组腹腔注射后24 h行尿流动力学...

Expression of store-operated calcium channels in bladder tissue of rats with cyclophosphamide induced overactive bladder

Objective To prepare rat models of overactive bladder(OAB)and to investigate the expression of store-operated calcium channels in the bladder tissue.Methods Thirty healthy female SD rats were randomly and equally divided into model group and control group.OAB models were established by intraperitoneal single injection of cyclophosphamide 200mg/kg in model group,and control group was intraperitoneally injected with the same amount of normal saline.Urodynamic detection was done 24hafter intraperitoneal injection to observe the voiding interval,voiding threshold,basal bladder pressure and mean detrusor pressure.The rats were sacrificed immediately after urodynamic detection to obtain the bladder tissue.Western blot was used to detect the expressions of calcium release activated calcium channel protein 1(Orai1)and stromal interacting molecule 1(STIM1)proteins in the bladder tissue.Real-time fluorescence quantitative PCR was used to detect the relative expressions of Oria1mRNA and STIM1mRNA.Results In control group,the detrusor contractions were stable during the period of urine storage and urination,and no obvious unstable contractions were found.In model group,obvious unstable contractions occurred during the period of urine storage.The voiding interval was shorter in model group((217.8±176.7)s)than that in control group((412.8±276.4)s)(P<0.05),the voiding threshold was lower in model group((10.0±2.6)cm H₂O)than that in control group((14.3±2.7)cm H₂O)(P<0.05),the basal bladder pressure was higher in model group((11.4±3.7)cm H₂O)than that in control group((7.5±2.6)cm H₂O)(P<0.05),and the mean detrusor pressure showed no significant difference between model group((54.8±20.7)cm H₂O)and control group((43.2±8.2)cm H₂O)(P>0.05).The relative expressions of Orai1and STIM1proteins and mRNAs were higher in model group(0.36±0.21,0.63±0.46,1.92±0.80,0.42±0.22)were higher than those in control group(0.21±0.13,0.44±0.31,0.59±0.06,0.16±0.02)(P<0.05).Conclusion The expressions of store-operated calcium channels related proteins Orai1and STIM1increase in OAB rat models.