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| 关木通两种提取液对V79细胞DNA的损伤作用 | |
| 引用本文： | 刘斌,艾刚,岑小波,陈波,王莉,刘玉清. 关木通两种提取液对V79细胞DNA的损伤作用[J]. 华西药学杂志, 2003, 18(1): 13-16 |
| 作者姓名： | 刘斌 艾刚 岑小波 陈波 王莉 刘玉清 |
| 作者单位： | 1. 四川大学华西医院,国家,成都,中药安全性评价中心,四川,成都,610041;四川大学公共卫生学院,四川,成都,610044 2. 四川大学华西医院,国家,成都,中药安全性评价中心,四川,成都,610041 3. 四川大学公共卫生学院,四川,成都,610044 |
| 摘 要： | 目的 探讨关木通对DNA的损伤作用。方法 应用单细胞凝胶电泳技术（彗星实验）研究中药关木通的两种提取液对中国仓鼠肺成纤维细胞（V79）的DNA损伤情况。结果 关木通的两种提取液在一定浓度下均能导致V79细胞产生彗星现象，并且存在的剂量-反应关系。结论 提示关木通过V79细胞DNA具有损伤作用。 |
| 关 键 词： | 关木通 氨水－乙醇提取液 水煎液 中药 不良反应 肺成纤维细胞 DNA损伤 单细胞凝胶电泳技术 动物实验 |
| 文章编号： | 1006-0103(2003)01-0013-04 |
| 修稿时间： | 2002-11-01 |
Study on the DNA damage of V79 cells by two kinds of extracts from Aristolochia Manshuriensis Kom |
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| LIU Bin ,,AI Gang ,CEN Xiao-bo ,CHEN Bo ,WANG Li ,LIU Yu-qing .West China Hospital of Sichuan University,National Center for Safety Evaluation of Traditional Chinese Medicine,Chengdu ,China .School of Public Health,Sichuan University,Chengdu ,China. Study on the DNA damage of V79 cells by two kinds of extracts from Aristolochia Manshuriensis Kom[J]. West China Journal of Pharmaceutical Sciences, 2003, 18(1): 13-16 | |
| Authors: | LIU Bin AI Gang CEN Xiao-bo CHEN Bo WANG Li LIU Yu-qing .West China Hospital of Sichuan University National Center for Safety Evaluation of Traditional Chinese Medicine Chengdu China .School of Public Health Sichuan University Chengdu China |
| Affiliation: | LIU Bin 1,2,AI Gang 1,CEN Xiao-bo 1,CHEN Bo 1,WANG Li 1,LIU Yu-qing 21.West China Hospital of Sichuan University,National Center for Safety Evaluation of Traditional Chinese Medicine,Chengdu 610041,China 2.School of Public Health,Sichuan University,Chengdu 610044,China |
| Abstract: | OBJECTIVE To study the DNA damage induced by Aristolochia Manshuriensis Kom..METHODS The DNA damage of V 79 cell induced by extracts from Aristolochia Manshuriensis Kom.was studied in vitro with single cell electrophoresis assay (comet assay). RESULTS It showed that the extracts could induce V 79 cell to form comet at a certain dose,and there existed the dose-response relationship.CONCLUSION It suggested that Aristolochia Manshuriensis Kom.can cause DNA damage. |
| Keywords: | Aristolochia Manshuriensis Kom V79cells DNA damage Comet assay |
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