DNA依赖蛋白激酶在鼻咽癌病变中的表达及意义

目的]研究DNA依赖蛋白激酶(DNA-PKcs)在鼻咽部正常黏膜、鼻咽炎、鼻咽非典型增生及鼻咽癌中的表达,并探讨DNA-PKcs表达与鼻咽癌临床病理参数间的关系.方法]应用免疫组化EnVision法检测10例鼻咽部正常黏膜、10例鼻咽炎、21例鼻咽非典型增生和53例鼻咽癌组织中DNA-PKcs蛋白的表达水平,并结合临床病理特征进行分析.结果]DNA-PKcs蛋白在正常鼻咽黏膜及鼻咽炎组织中呈低表达,在鼻咽非典型增生及鼻咽癌组织中呈高表达,阳性表达率分别为0、20.00％、71.42％和64.15％;其表达与鼻咽癌组织分化类型相关(P=0.023),与性别、年龄、临床分期及淋巴结转移无明显相关性(P均＞0.05).结论]DNA-PKcs在鼻咽癌病变过程中可能发挥一定的作用.

Expression and Clinical Significance of DNA-dependent Protein Kinase in Nasopharyngeal Carcinoma

Purpose] To investigate the expression of DNA-dependent protein kinase(DNA-PKcs) in normal nasopharyngeal mucosa,nasopharyngitis,nasopharyngeal atypical hyperplasia and nasopharyngeal carcinoma(NPC),and to analysis the relationship between DNA-PKcs protein and the clinicopathologic parameters of NPC.Methods] The expression of DNA-PKcs protein in tissue samples of nasopharyngeal mucosa (n=10),nasopharyngitis (n=10),nasopharyngeal atypical hyperplasia(n=21) and NPC(n=53) was detected by immunohistochemical technique(Envision).The relationship between DNA-PKcs expression and clinicopathological features of NPC was analyzed.Results] The positive rates of DNA-PKcs in normal nasopharyngeal mucosa,nasopharyngitis,nasopharyngeal atypical hyperplasia and NPC were 0,20.00％,71.42％ and 64.15％,respectively.DNA-PKcs expression was correlated with tumor differentiation (P＜0.05),but not correlated with clinical stages or neck lymph node metastasis of NPC (P＞0.05).Conclusion] DNA-PKcs may be involved in the carcinogenesis of nasopharyngeal neoplasms.