洋金花茎化学成分的分离鉴定

目的:研究洋金花Datura metel茎的化学成分。方法:采用硅胶,ODS柱色谱及HPLC等色谱分离方法进行分离纯化,利用NMR,HR-ESI-MS等波谱学方法结合参考文献鉴定化合物的结构。结果:从洋金花茎70%乙醇提取物的乙酸乙酯萃取部位中分离得到14个化合物,分别鉴定为N-[2-(3,4-dihydroxyphenyl-2-hydroxyethyl)]-3-(4-methoxyphenyl)prop-2-enamide(1),3-(4-hydroxy-3-methoxyphenyl)-N-[2-(4-hydroxyphenyl)-2-methoxyethyl]acrylamide(2),N-反式-对-香豆酰基章鱼胺(3),N-顺式阿魏酰基酪胺(4),N-trans-feruloyl-3',4'-dihydroxyphenylethylamine(5),N-反式阿魏酰基酪胺(6),N-反式-对-香豆酰基酪胺(7),草夹竹桃苷(8),daturataturin A(9),baimantuoluoside H(10),daturametelin A(11),柑橘苷A(12),9,12,13-三羟基-10,15-十八碳二烯酸甲酯(13),(9E)-8,11,12-三羟基-十八碳烯酸甲酯(14)。结论:化合物1,8为首次从茄科植物中分离得到,化合物2~5为首次从曼陀罗属植物中分离得到。

Isolation and Identification of Chemical Constituents from Stems of Datura metel

Objective: To study the chemical constituents from the stems of Datura metel. Method: The compounds were isolated and purified by silica gel and ODS chromatogram columns together with semi-preparative HPLC. The structures of the isolated compounds were identified by ¹H-NMR and ¹³ C-NMR as well as HR-ESI-MS data. Result: Fourteen compounds were isolated from the ethyl acetate fraction of the 70% ethanol extract from the stems of D. metel, which were identified as N-[2-(3,4-dihydroxyphenyl-2-hydroxyethyl)]-3-(4-methoxyphenyl) prop-2-enamide (1),3-(4-hydroxy-3-methoxyphenyl)-N-[2-(4-hydro-xyphenyl)-2-methoxyethyl] acrylamide (2),N-trans-p-coumaroyloctopamine (3), N-cis-feruloyltyramine (4),N-trans-feruloyl-3'',4''-dihy-droxyphenylethylamine (5),N-trans-feruloyl tyramine (6), trans-N-p-coumaroyltyramine (7),androsin (8),daturataturin A (9),baimantuoluoside H (10), daturametelin A (11), citroside A (12), methyl-9,12,13-trihydroxyoctadeca-10,15-dienoate (13) and (9E)-8,11,12-trihydroxyoctadecenoic acid methyl ester (14). Conclusion: Compounds 1 and 8 were isolated from the plants of Solanaceae for the first time and compounds 2 - 5 were isolated from Datura genus for the first time.