| 薏苡叶总碱抗氧化研究 |
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| 引用本文： | 杨海梅,黄凯敏,苏汶君,覃春滤,黄锁义. 薏苡叶总碱抗氧化研究[J]. 微量元素与健康研究, 2014, 0(1): 22-23 |
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| 作者姓名： | 杨海梅 黄凯敏 苏汶君 覃春滤 黄锁义 |
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| 作者单位： | [1]右江民族医学院药学院,广西百色533000 [2]右江民族医学院公共卫生与管理学院,广西百色533000 [3]右江民族医学院护理学院,广西百色533000 |
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| 基金项目： | 国家自然科学基金资助项目（No.81360684）;广西自然科学基金资助课题（2011GXNSFA018046）;广西中医药科技专项资助课题（GZKZ10-128）;广西中医药管理局课题（gzze1047）;广西医学科学实验中心开放基金专项资助项目（KFJJ2011-04）;广西自治区级大学生创新训练计划立项资助项目（QJCX201203）;右江民族医学院2010-2012年度普高学生科研项目（右医科字[2010]9号） |
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| 摘 要： | 目的:研究广西薏苡叶总碱的抗氧化性,为薏苡叶的开发利用提供参考依据。方法:以75%乙醇为溶剂提取薏苡叶中的总碱类物质,采取比色法测定薏苡叶总碱对DPPH·、O2-和·OH 3三种自由基的清除作用以及对Fe3+还原能力。结果:薏苡叶有较强的清除作用及还原Fe3+能力。结论:清除率与浓度存在明显的量效关系,当总碱浓度为0.50 mg/ml时,其清除率O2-·为43.5%,DPPH·为69.2%,·OH为11.2%;还原Fe3+能力随总碱的浓度增加而增强。
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| 关 键 词： | 薏苡叶 总碱 抗氧化 清除率 |
Study on antioxidant effect of total alkaloids from the Coixlachryma- jobi L. leaves |
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| Affiliation: | YANG Hai - mei, HUANG Kai - min, SU Wen - jun, QIN Ohun - lv, HUANG Suo - yi (1. College of Pharmacy Youjiang Medical University for Nationalities; 2. College of Public Health and Management, Youfiang Medical University for Nationalities ; 3. College of Nursing, Youjiang Medical University for Nationalities ： Baise Guangxi 533000, China ) |
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| Abstract: | Objective： sturdy on the antioxidant activity of total alkaloids in the Coixlachryma- jobi L. leaves, to make full. use of Coixlachryma- jobiL, leaves resources. Methods： The alkaloids were extracted with w （Ethanol） = 75 % as the solvent from Coixlaehryma- jobi L. leaves. It will determine the total alkaloid＇s scavenging process acting on DPPH·,-OH and O2^- · with the Spectrophotometry, and the reduction of Fe^3＋ ability. Results： Total alkaloids from Coixlachryma - jobi L. leaves have significant scavenging effect to three kinds of free radicals , and the reduction of Fe^3＋ ablity. Concision： Clearance and concentration obvious concentrationresponse relationship. When the content of the total alkaloids from Coixlachryma- jobi L. leaves was ρ（Flavanone） = 0. 10mg/mL, the clearance rate of DPPH· , · OH and O2^- · was 69.2 % , 11.2 % and 43.5 %. Eviden reduction energy of the flavanone was increansing by the concentration. |
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| Keywords: | Coixlachryma - jobi L. leaves total alkaloids antioxidan clearance |
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