螺内酯对心肌梗死大鼠血流动力学及血清TGF-β1的影响

目的 研究螺内酯对大鼠心肌梗死后血清转化生长因子-β1(TGF-β1)的影响.方法 结扎SD大鼠冠状动脉前降支,建立心肌梗死模型,将术后存活24 h大鼠随机分为两组:螺内酯(MIS)组给予螺内酯20 mg/(kg·d)溶于2 ml生理盐水灌胃,连续4周;心肌梗死(MI)对照组给予等体积的生理盐水,穿针但不结扎前降支的作为假手术组,给予等体积的生理盐水,4周末行血流动力学测定,同时断尾取血测血清TGF-β1含量.结果 与假手术组相比,4周末AMI组血清TGF-β1含量显著升高(P＜0.01),左室舒张末压(LVEDP)显著增加(P＜0.01),左室内压最大收缩速率(+dp/dtmax)显著下降(P＜0.01).与AMI组相比,4周末螺内酯组血清TGF-β1含量显著下降(P＜0.01),LVEDP显著下降(P＜0.01),而+dp/dtmax显著增加(P＜0.01).结论 螺内酯可能通过抑制TGF-β1产生而改善心功能,抑制心肌纤维化. Abstract： Objective To observe the effects of aldactone on blood serum TGF - β1 in acute myocardial infarction rats. Methods Rats surviving 24 h after AMI induced by left anterior descending branch ligation were randomized to aldactone and control group compared with sham - operated group. The aldactone group was given aldactone 20 mg/( kg · d) , the MI control group and sham -operated group were given an equal volume of saline, continued for 4 weeks. At the end of 4th week,the hemodynamics change was detected, then tails to take blood and measure the serum TGF - β1.Results Compared with the sham - operated rats,the serum TGF - β1 level and the LVEDP of the AMI rats were significantly elevated at the end of the 4th week ( P ＜0.01 ) , while the + dp/dtmax were significantly reduced ( P ＜0.01 ) compared with the AMI control group, the serum TGF - β1 level and the LVEDP of the aldactone group rats decrease significantly at the end of the 4th week (P ＜ 0.01 ) ,while the + dp/dtmax were significantly elevated ( P ＜ 0.01 ). The difference had statistical significance. Conclusions Aldactone may improve the cardiac fibrosis and inhibit myocardial fibrosis by inhibiting the production of the TGF - β1.