食管鳞癌组织中缺氧诱导因子-1α和6-氧-甲基鸟嘌呤-DNA甲基转移酶蛋白的表达与预后的关系

目的 研究食管鳞状细胞癌(ESCC)中缺氧诱导因子-1α(HIF-1α)、6-氧-甲基鸟嘌呤-DNA甲基转移酶(MGMT)蛋白的表达及其与食管癌病理特征的关系.方法 采用免疫组织化学(SP)法检测58例食管鳞癌组织中HIF-1α、MGMT蛋白的表达,并分析其与临床病理各因素之间的关系.结果 58例患者的ESCC组织中HIF-1α、MGMT蛋白均有表达,阳性表达率分别为51.4%、39.6%,MGMT 蛋白的表达与食管鳞癌的分化程度密切相关(P<0.05),而与性别、年龄、分期及淋巴结转移无关.HIF-1α蛋白的表达与性别、年龄、分期、分化程度以及淋巴结转移均无关(P均>0.05).HIF-1α与MGMT 蛋白表达无明显相关关系(P>0.05).MGMT阳性表达患者生存时间(OS)较阴性组明显延长(P<0.05),而HIF-1α蛋白表达与OS无明显相关(P>0.05).结论 HIF-1α蛋白在食管鳞癌组织中呈高表达,MGMT与其分化程度相关,两者检测对判断预后有重要意义. Abstract： Objective To investigate the expression of hypoxia-inducible factor-1α (HIF-1α) and O~6-methylguanine DNA methytransferase(MGMT) in esophageal squamous cell carcinoma(ESCC) tissue and its relation with the clinicopathalogy features. Methods The expression of HIF-1α, MGMT in 58 cases of ESCC were detected by immnohistochemical staining. Results The positive rates of HIF-1α,MGMT in ESCC tissue were 51.4%,39.6%, respectively. The positive expression rates of HIF-1α and MGMT were not correlated with the degree of sex,age,clinical stage and differentiation(P>0.05), but MGMT expression was correlated with differentiation and overall survival time (P<0.05). The expression of HIF-1α was not correlated with that of MGMT (P>0.05), and HIF-1α was not correlated with overall survival time. Conclusions HIF-1α, MGMT play important roles in esophageal carcinoma,MGMT was correlated with the differentiation of esophageal carcinoma, respectively, which may be used as important indexes to evaluate the prognosis of esophageal carcinoma.

Expression of hypoxia-inducible factor-1 and O~6-methylguanine DNA methytransferase in esophageal squamous cell carcinoma tissue

Esophageal neoplasm Squamous cell carcinoma Hypoxia-inducible factor-1 O~6-methylguanine DNA methytransferase