|
|||||
|
|
| 大剂量葡萄糖诱导人视网膜内皮细胞凋亡的实验研究 | |
| 引用本文： | 马建芳,杨中汉,宋志宏,郭颖,蔡卫斌,刘倩平,郭琳琅,高国全. 大剂量葡萄糖诱导人视网膜内皮细胞凋亡的实验研究[J]. 南方医科大学学报, 2003, 23(5): 435-438 |
| 作者姓名： | 马建芳 杨中汉 宋志宏 郭颖 蔡卫斌 刘倩平 郭琳琅 高国全 |
| 作者单位： | 1. 解放军第476医院内科,福建,福州,350002 2. 中山大学中山医学院生物化学教研室,广东,广州,510089 3. 第一军医大学珠江医院内科,广东,广州,510282 4. 第一军医大学珠江医院病理科,广东,广州,510282 |
| 基金项目： | 教育部回国人员启动基金(2003)，中山大学回国人员启动基金~~ |
| 摘 要： | 目的检验大剂量葡萄糖水平对培养的原代人视网膜毛细血管内皮细胞(humanretinalcapillaryendothelialcells,HRCEC)的直接影响。方法HRCEC细胞从捐赠人眼中分离得到。细胞在含有5mmol/L或25mmol/L葡萄糖的培养基中培养6d。应用锥虫蓝染色测定细胞活力、流式细胞计数分析细胞周期、TUNEL分析细胞凋亡。结果HRCEC细胞在25mmol/L葡萄糖中培养6d,细胞活力显著下降。通过流式细胞计数和TUNEL测定,凋亡细胞的数目在大剂量葡萄糖培养基中显著升高。结论大剂量葡萄糖可诱导人视网膜毛细血管内皮细胞凋亡,这有可能是促进糖尿病性视网膜病发展的原因。 |
| 关 键 词： | 视网膜病 糖尿病性 凋亡 视网膜内皮细胞 高血糖症 流式细胞计数 |
| 文章编号： | 1000-2588(2003)05-0435-04 |
| 修稿时间： | 2002-12-21 |
High-dose glucose induces human retinal endothelial cell apoptosis |
|
| MAJian-fang,YANGZhong-han,SONGZhi-hong,GUOYin,CAIWei-bin,LIUQian-ping,GUOLin-lang,GAOGuo-quanDepartmentofInternalMedicine,ArmyHospital,Fuzhou,,China,DepartmentofBiochemistry,SunYat-senMedicalCollegeofSunYat-SenUniversity,Guangzhou,China,DepartmentsofInternalMedicineandPathology,ZhujiangHospital,FirstMilitaryMedicalUniversity,Guangzhou,China. High-dose glucose induces human retinal endothelial cell apoptosis[J]. Journal of Southern Medical University, 2003, 23(5): 435-438 | |
| Authors: | MAJian-fang YANGZhong-han SONGZhi-hong GUOYin CAIWei-bin LIUQian-ping GUOLin-lang GAOGuo-quanDepartmentofInternalMedicine ArmyHospital Fuzhou China DepartmentofBiochemistry SunYat-senMedicalCollegeofSunYat-SenUniversity Guangzhou China DepartmentsofInternalMedicineandPathology ZhujiangHospital FirstMilitaryMedicalUniversity Guangzhou China |
| Affiliation: | MAJian-fang1,YANGZhong-han2,SONGZhi-hong2,GUOYin3,CAIWei-bin2,LIUQian-ping2,GUOLin-lang4,GAOGuo-quan21DepartmentofInternalMedicine,476ArmyHospital,Fuzhou,350002,China,2DepartmentofBiochemistry,SunYat-senMedicalCollegeofSunYat-SenUniversity,Guangzhou510089,China,DepartmentsofInternalMedicine3andPathology4,ZhujiangHospital,FirstMilitaryMedicalUniversity,Guangzhou520282,China |
| Abstract: | Objective To examine the direct effect of high glucose le ve ls on primary cultured human retinal capillary endothelial cells (HRCEC). Methods HRCECs were isolated from donated eyes and cultured for 6 days in the m edia containing 5 or 25 mmol/L glucose. The cell viability was determined by try pan blue exclusion assay and cell cycle analyzed by flow cytometry, with the ce ll apoptosis assayed by TUNEL method. Results The cell viability was significant ly decreased after exposure to 25 mmol/L glucose, and the number of apo ptotic cells determined by flow cytometry and TUNEL was significantly increased in response to high-dose glucose treatment. Conclusion High-dos e glucose induces apoptosis in HRCEC, which may contribute to the developme nt of diabetic retinopathy. |
| Keywords: | retinopathy diabetic apoptosis retinal e ndothelial cells hyperglycemia flow cytometry |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |