应用原位免疫聚合酶链反应检测活动性巨细胞病毒感染

目的建立一种新的诊断活动性巨细胞病毒感染的方法。方法应用原位免疫聚合酶链反应(PCR)技术检测早、中、晚期孕妇宫颈脱落细胞中巨细胞病毒抗原。结果119例受检标本中有16例出现人巨细胞病毒(HCMV)抗原阳性，孕早、中、晚期孕妇活动性HCMV感染率分别为：9.37%、11.90%、17.77%；检出的HCMV抗原阳性细胞数为2～38／5万宫颈脱落细胞，平均8.5／5万细胞。常规ABC法平行检测，原位免疫PCR法检测敏感性高于ABC法。结论原位免疫PCR方法敏感性高、特异性强，可检出微量HCMV抗原，为临床诊断活动性HCMV感染提供一种新方法。

Detecion of Active Human Cytomegalovirus Infection by Immuno-in situ PCR Assay

OBJECTIVE To establish a new method for the diagnosis of activehuman cytomegalovirus(HCMV) infection.METHODS Immuno-in situ PCR was used for detection of HCMV antigen in pregnant women cervix uteri exfoliated cells.RESULTS Among 119 pregnant women,the rate of active HCMV infection during the first,second and third trimesters of gestation was 9.37%,11.90%,17.77%,respectively.HCMV antigen positive cells were counted in every 5.0×104 exfoliated cells of cervix uteri.The average count of HCMV antigen positive cells in active infection of pregnant women was (8.5-5.0)×104 cells.The sensitivity of immuno-in situ PCR was significantly higher than that of ABC.CONCLUSIONS The method of immuno-in-situ PCR is sensitive and specific,it can be applied in clinic to diagnose active HCMV infection.