神经源性分化因子在新生大鼠短暂脑缺氧中的表达

目的 观察新生大鼠短暂性缺氧后脑神经源性分化因子(NeuroD)表达的变化.方法 新生10～24h的SD大鼠短暂置于100%氮气环境中,建立缺氧窒息模型.33只新生大鼠随机分为对照组、缺氧10min组、缺氧20min组,采用免疫荧光法和Western blotting检测NeuroD表达的变化.结果 在大脑皮质区和海马齿状回可见NeuroD免疫荧光染色阳性细胞;Western blotting分析显示,随缺氧时间的延长,NeuroD的表达量明显增加,缺氧10min组与对照组、缺氧20min组与缺氧10min组比较,差异有统计学意义(P<0.01).结论 短暂缺氧可引起NeuroD表达量的增加,表达部位主要在大脑皮质、海马等神经干细胞/神经前体细胞聚集区.

Expression of neurogenic differentiation factor in the brain of transient hypoxia postnatal rats

Objective To observe the effects of transient hypoxia in the brain on the expression level of neurogenic differentiation factor (NeuroD). Methods The model was established as previously described by Grojean. Briefly, newborn rats were transiently exposed to 100% NSUB>2/SUB>.Thirty-three rats were randomly divided into the normal control group, 10min hypoxia group and 20min hypoxia group. The expression level of NeuroD in a rat brain was determined with Western blotting and immunofluorescence staining. Results Immunofluorescence analysis showed that NeuroD was expressed in the cerebral cortex and dentate gyrus. Western blotting analysis demonstrated a significant up regulation in the NeuroD expression with the time after hypoxia. The expression of NeuroD significantly increased in the 10min hypoxia group compared to the control group ( EM>P/EM> <0.01), with the highest expression level being seen in the 20min hypoxia group (compared to 10min hypoxia, EM> P/EM> <0.01). BR> Conclusion Transient hypoxia induces the up-regulation of NeuroD, which was found to express mainly in neural stem /progenitor cells rich areas, i