脑缺血再灌注大鼠半暗带皮质谷氨酸受体相互作用蛋白表达的增加

目的 探讨脑缺血再灌注大鼠半暗带皮质中谷氨酸受体相互作用蛋白(GRIP)表达变化的规律.方法 SD雄性大鼠,随机分为脑缺血2h后再灌注1、3、6、12、24、72h组和假手术组(每组6只大鼠),采用线栓-再通法制作大脑中动脉闭塞模型,于再灌注后相应时间点处死大鼠取脑,免疫组织化学方法观察GRIP 在皮质中的表达及分布,...

Increase of glutamate receptor interacting protein in the penumbra cortex of rats after transient ischemia and reperfusion

Objective To explore the expression of glutamate receptor interacting protein (GRIP) after ischemia-reperfusion in the rat brain. Methods Seventy-two male Sprague Dawley rats were randomly divided into 6 ischemia-reperfusion groups and sham groups with 6 rats per group. Middle cerebral artery occlusion (MCAO) was achieved with the use of an intraluminal filament to occlude the left middle cerebral artery, and reperfused 2 hours later. At the 1st, 3rd, 6th, 12th, 24th and 72th hour after reperfusion or sham operation, the rats from each group were decapitated. Immunohistochemistry and double immunofluorescence were used to observe the distribution of GRIP in the cortex and also its relationship with glutamate receptor 2 (GluR2). Results Compared with the sham-operated group which showed few positive neurons, GRIP positive neurons increased obviously from 3 hours after ischemia-reperfusion in the ischemic penumbra cortex, mainly on the dendrites at the early stage and moved to the cell body lately. Double immunohistochemical staining showed that the GRIP positive cells were co-stained with GluR2 neurons. Conclusion GRIP increased and accelerated to transfer GluR2 to the membrane after ischemia-reperfusion which may protect the neurons in the penumbra zone from the AMPA receptor mediated excitotoxic injury.