白藜芦醇调控癫痫持续状态大鼠海马内源性巯醇抗氧化剂(酶)的作用

目的 探讨白藜芦醇(Res)在氯化锂联合重复低剂量匹罗卡品诱导癫痫持续状态大鼠模型中的抗氧化作用及其机制.方法 54只SD大鼠随机分为 3 组:对照组、癫痫模型组和Res治疗组,采用氯化锂联合重复低剂量匹罗卡品腹腔注射制备癫痫持续状态大鼠模型,Res治疗组同时给予白藜芦醇预防性治疗.对大鼠按痫性分级标准进行行为学观测;...

Resveratrol regulates endogenous thiol antioxidants of the hippocampal in status epilepticus rat model

Objective To explore the antioxidatition and mechanism of the resveratrol’s in status epilepticus（SE）rat model induced by treated with LiCl plus repeated low doses of pilocarpine. Methods Fifty-four SD rats were divided into three groups at random: control group, model group and treatment group. The rat SE model was established by injecting LiCl plus repeated low doses of pilocarpine into the abdominal cavity. Meanwhile, model rats were treated by injecting peritoneally resveratrol (30mg/kg). The concentration of the thiol antioxidants, including superoxide dismutase (SOD), glutathione (GSH), glutathione peroxidase (GSH-PX) and glutathione reductase (GR), were examined by the colorimetric method in the hippocampus. The protein expression of the Bcl-2, Caspase-3 and Bax were measured using the immunohistochemistry and Western blotting technology. Results The SE model rats exhibited a significant decrease in the concentration of SOD, GSH, GSH-PX, GR and Bcl-2, while an increase in concentration of Bax, Caspase-3 and the number of positive cells compared to the control group ( P 0.05). After treatment of the resveratrol, the treatment group rats exhibited significant an increase in seizure latency. Resveratrol significantly up regulated the concentration of the thiol antioxidants (SOD, GSH, GSH-PX, GR) and increased the expression of the Bcl-2 and the number of the positive cells ( EM>P /EM>0.05), while decreasing the expression of Caspase-3, Bax and the number of the positive cells compared to model group ( EM>P/EM> 0.05). Conclusion Resveratrol may resist the oxidative damage induced by SE, and play a neuroprotective role in the hippocampus of the SE model rat by up regulating t